The catalytic activity of Ag2S-montmorillonites as peroxidase mimetic toward colorimetric detection of H2O2

Qingyun Liu; Yanling Jiang; Leyou Zhang; Xinpei Zhou; Xintian Lv; Yanyuan Ding; Lifang Sun; Pengpeng Chen; Hailiang Yin

doi:10.1016/j.msec.2016.04.007

The catalytic activity of Ag2S-montmorillonites as peroxidase mimetic toward colorimetric detection of H2O2

Mater Sci Eng C Mater Biol Appl. 2016 Aug 1:65:109-15. doi: 10.1016/j.msec.2016.04.007. Epub 2016 Apr 4.

Authors

Qingyun Liu¹, Yanling Jiang², Leyou Zhang², Xinpei Zhou², Xintian Lv³, Yanyuan Ding², Lifang Sun², Pengpeng Chen², Hailiang Yin⁴

Affiliations

¹ School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China. Electronic address: qyliu@sdust.edu.cn.
² School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
³ School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, PR China.
⁴ Academy of Science & Technology, China University of Petroleum, Dongying 257061, PR China.

PMID: 27157733
DOI: 10.1016/j.msec.2016.04.007

Abstract

Nanocomposites based on silver sulfide (Ag2S) and Ca-montmorillonite (Ca(2+)-MMT) were synthesized by a simple hydrothermal method. The nanocomposites were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectra (FTIR). The as-prepared Ag2S-MMT nanocomposites were firstly demonstrated to possess intrinsic peroxidase-like activity and could rapidly catalytically oxidize the substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a blue product which can be seen by the naked eye in only one minute. The experimental results revealed that the Ag2S-MMT nanocomposites exhibit higher thermal durance. Based on the TMB-H2O2 catalyzed color reaction, the Ag2S-MMT nanocomposites were exploited as a new type of biosensor for detection and estimation of H2O2 through a simple, cheap and selective colorimetric method.

Keywords: Ag(2)S; Ca-montmorillonite; Colorimetric; H(2)O(2); Peroxidase.

MeSH terms

Animals
Bentonite / chemistry*
Biocompatible Materials / chemistry
Biocompatible Materials / metabolism*
Biosensing Techniques
Catalysis
Colorimetry*
Hydrogen Peroxide / analysis*
Limit of Detection
Microscopy, Electron, Transmission
Milk / chemistry
Nanocomposites / chemistry
Oxidation-Reduction
Peroxidase / metabolism
Silver Compounds / chemistry*
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction

Substances

Biocompatible Materials
Silver Compounds
Bentonite
silver sulfide
Hydrogen Peroxide
Peroxidase