In vitro selection and amplification protocols for isolation of aptameric sensors for small molecules

Kyung-Ae Yang; Renjun Pei; Milan N Stojanovic

doi:10.1016/j.ymeth.2016.04.032

In vitro selection and amplification protocols for isolation of aptameric sensors for small molecules

Methods. 2016 Aug 15:106:58-65. doi: 10.1016/j.ymeth.2016.04.032. Epub 2016 May 4.

Authors

Kyung-Ae Yang¹, Renjun Pei², Milan N Stojanovic³

Affiliations

¹ Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, United States.
² Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, China.
³ Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, United States; Department of Biomedical Engineering, Columbia University, New York, NY 10032, United States. Electronic address: mns18@cumc.columbia.edu.

Abstract

We recently optimized a procedure that directly yields aptameric sensors for small molecules in so-called structure-switching format. The protocol has a high success rate, short time, and is sufficiently simple to be readily implemented in a non-specialist laboratory. We provide a stepwise guide to this selection protocol.

Keywords: Aptamer; Biosensor; SELEX; Small molecules; Structure-switching.

Publication types

Review
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Aptamers, Nucleotide / chemistry
Aptamers, Nucleotide / genetics*
Biosensing Techniques / methods*
Nucleic Acid Conformation
SELEX Aptamer Technique / methods*
Small Molecule Libraries / chemistry

Substances

Aptamers, Nucleotide
Small Molecule Libraries

Grants and funding

R01 GM104960/GM/NIGMS NIH HHS/United States