Amino acid analysis for pharmacopoeial purposes

Oliver Wahl; Ulrike Holzgrabe

doi:10.1016/j.talanta.2016.03.071

Amino acid analysis for pharmacopoeial purposes

Talanta. 2016 Jul 1:154:150-63. doi: 10.1016/j.talanta.2016.03.071. Epub 2016 Mar 22.

Authors

Oliver Wahl¹, Ulrike Holzgrabe²

Affiliations

¹ University of Würzburg, Institute for Pharmacy and Food Chemistry, 97074 Würzburg, Germany.
² University of Würzburg, Institute for Pharmacy and Food Chemistry, 97074 Würzburg, Germany. Electronic address: ulrike.holzgrabe@uni-wuerzburg.de.

PMID: 27154660
DOI: 10.1016/j.talanta.2016.03.071

Abstract

The impurity profile of amino acids depends strongly on the production process. Since there are many different production methods (e.g. fermentation, protein hydrolysis or chemical synthesis) universal, state of the art methods are required to determine the impurity profile of amino acids produced by all relevant competitors. At the moment TLC tests provided by the Ph. Eur. are being replaced by a very specific amino acid analysis procedure possibly missing out on currently unknown process related impurities. Production methods and possible impurities as well as separation and detection methods suitable for said impurities are subject to this review.

Keywords: Amino acids; Detection; Impurity profiling; Production; Separation.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Amino Acids / analysis*
Drug Contamination

Substances

Amino Acids