One of the most widely used tools in epidemiological research on discrimination is the Experiences of Discrimination (EOD) scale, used but not validated in Brazil. The objective was to assess the reliability and dimensional structure of the EOD scale in a Brazilian population. A cross-sectional study was performed with 1,380 adults in the city of Salvador, Bahia State, Brazil. Confirmatory factor analysis (CFA) was performed testing a two-factor model: experiences of discrimination and concerned about discrimination. The results of CFA showed satisfactory fit, high factor loads, and adequate reliability, confirming the scale's internal consistency. Residual correlations were identified involving items from both factors. The dimensional structure presented in this study highlights the importance of using different measures of discrimination (interpersonal and group) to allow more in-depth future research on the effects of racism on health.