A next-generation proteome array for Schistosoma mansoni

Int J Parasitol. 2016 Jun;46(7):411-5. doi: 10.1016/j.ijpara.2016.04.001. Epub 2016 Apr 27.

Abstract

A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.

Keywords: Antibody signature; Protein microarray; Proteome; Schistosoma mansoni.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Helminth / immunology
  • Antigens, Helminth / chemistry
  • Antigens, Helminth / genetics
  • Antigens, Helminth / immunology
  • Computational Biology
  • Helminth Proteins / chemistry
  • Helminth Proteins / genetics*
  • Helminth Proteins / immunology
  • Immune Sera / immunology
  • Immunoglobulin G / immunology
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics
  • Membrane Proteins / immunology
  • Pilot Projects
  • Protein Array Analysis* / methods
  • Proteome / chemistry*
  • Proteome / genetics
  • Proteome / immunology
  • Schistosoma mansoni / chemistry*
  • Schistosoma mansoni / genetics
  • Schistosoma mansoni / immunology
  • Schistosomiasis mansoni / immunology*
  • Schistosomiasis mansoni / parasitology

Substances

  • Antibodies, Helminth
  • Antigens, Helminth
  • Helminth Proteins
  • Immune Sera
  • Immunoglobulin G
  • Membrane Proteins
  • Proteome