Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

Dominique T Monteil; Xiao Shen; Giulia Tontodonati; Lucia Baldi; David L Hacker; Florian M Wurm

doi:10.1016/j.ab.2016.04.009

Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

Anal Biochem. 2016 Jul 15:505:26-8. doi: 10.1016/j.ab.2016.04.009. Epub 2016 Apr 27.

Authors

Dominique T Monteil¹, Xiao Shen¹, Giulia Tontodonati¹, Lucia Baldi¹, David L Hacker², Florian M Wurm³

Affiliations

¹ Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
² Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland; Protein Expression Core Facility (PECF), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
³ Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. Electronic address: florian.wurm@exellgene.com.

PMID: 27130502
DOI: 10.1016/j.ab.2016.04.009

Abstract

Disposable orbitally shaken TubeSpin bioreactor 600 tubes (TS600s) were recently developed for the bench-scale cultivation of animal cells in suspension. Here we compared batch cultures of Sf9 insect cells in TS600s, spinner flasks, and shake flasks. Superior cell growth was observed in TS600s and shake flasks as compared with spinner flasks, and more favorable oxygen-enriched cell culture conditions were observed in TS600s as compared with either spinner or shake flasks. The results demonstrated the suitability of TS600s as a disposable vessel for the cultivation of Sf9 cells in suspension.

Keywords: Cell growth; Disposable bioreactor; Gas transfer; Orbital shaking; Sf9 cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bioreactors*
Cells, Cultured
Sf9 Cells / cytology*
Suspensions

Substances

Suspensions