Biochemical and Structural Characterization of a Five-domain GH115 α-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T

J Biol Chem. 2016 Jul 1;291(27):14120-14133. doi: 10.1074/jbc.M115.702944. Epub 2016 Apr 18.

Abstract

Glucuronic acid (GlcAp) and/or methylglucuronic acid (MeGlcAp) decorate the major forms of xylan in hardwood and coniferous softwoods as well as many cereal grains. Accordingly, the complete utilization of glucuronoxylans or conversion to sugar precursors requires the action of main chain xylanases as well as α-glucuronidases that release the α- (1→2)-linked (Me)GlcAp side groups. Herein, a family GH115 enzymefrom the marine bacterium Saccharophagus degradans 2-40(T), SdeAgu115A, demonstrated activity toward glucuronoxylan and oligomers thereof with preference toward MeGlcAp linked to internal xylopyranosyl residues. Unique biochemical characteristics of NaCl activation were also observed. The crystal structure of SdeAgu115A revealed a five-domain architecture, with an additional insertion C(+) domain that had significant impact on the domain arrangement of SdeAgu115A monomer and its dimerization. The participation of domain C(+) in substrate binding was supported by reduced substrate inhibition upon introducing W773A, W689A, and F696A substitutions within this domain. In addition to Asp-335, the catalytic essentiality of Glu-216 was revealed by site-specific mutagenesis. A primary sequence analysis suggested that the SdeAgu115A architecture is shared by more than half of GH115 members, thus defining a distinct archetype for GH115 enzymes.

Keywords: GH115 α-glucuronidase; carbohydrate; carbohydrate degradation; catalytic apparatus; crystal structure; enzyme catalysis; enzyme domain composition; enzyme structure; protein domain.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Circular Dichroism
  • Gammaproteobacteria / enzymology*
  • Glycoside Hydrolases / chemistry
  • Glycoside Hydrolases / metabolism*
  • Marine Biology
  • Models, Molecular
  • Protein Conformation
  • Sequence Homology, Amino Acid

Substances

  • Glycoside Hydrolases
  • alpha-glucuronidase

Associated data

  • PDB/1K9E
  • PDB/1L4I
  • PDB/2VUR
  • PDB/3BWU
  • PDB/4H04
  • PDB/4ZMH