Driven Translocation of Polynucleotides Through an Aerolysin Nanopore

Anal Chem. 2016 May 17;88(10):5046-9. doi: 10.1021/acs.analchem.6b01514. Epub 2016 Apr 29.

Abstract

Aerolysin has been used as a biological nanopore for studying peptides, proteins, and oligosaccharides in the past two decades. Here, we report that wild-type aerolysin could be utilized for polynucleotide analysis. Driven a short polynucleotide of four nucleotides length through aerolysin occludes nearly 50% amplitude of the open pore current. Furthermore, the result of total internal reflection fluorescence measurement provides direct evidence for the driven translocation of single polynucleotide through aerolysin.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Toxins / chemistry*
  • DNA, Single-Stranded / analysis*
  • DNA, Single-Stranded / chemistry
  • Electrochemical Techniques
  • Fluoresceins / chemistry
  • Fluorescence
  • Fluorescent Dyes / chemistry
  • Polynucleotides / analysis*
  • Polynucleotides / chemistry
  • Pore Forming Cytotoxic Proteins / chemistry*
  • Pore Forming Cytotoxic Proteins / ultrastructure

Substances

  • Bacterial Toxins
  • DNA, Single-Stranded
  • Fluoresceins
  • Fluorescent Dyes
  • Polynucleotides
  • Pore Forming Cytotoxic Proteins
  • aerolysin