The goal was to revisit an important, yet unproven notion that accommodative microfluctuations facilitate the determination of direction (sign) of abrupt focus changes in the stimulus to accommodation. We contaminated the potential temporal cues from natural accommodative microfluctuations by presenting uncorrelated external (screen) temporal defocus noise that combined with the retinal image effects of natural microfluctuations. A polychromatic Maltese spoke pattern thus either modulated defocus at a combination of two temporal frequencies (on-screen noise condition) or was static (control condition). The on-screen conditions were combined with step changes in optical vergence that were randomized in direction and magnitude. Five subjects monocularly viewed stimuli through a Badal optical system in a Maxwellian view. An artificial 4-mm aperture was imaged at the entrance pupil of the eye. Wavefront aberrations were measured dynamically at 50 Hz using a custom Shack-Hartmann aberrometer. Dynamic changes in the Zernike defocus term with step changes in optical vergence were analyzed. We calculated the percentage of correct directional responses for 1, 2, and 3 D accommodative and disaccommodative step stimuli using preset criteria for latency, velocity, and persistence of the response. The on-screen noise condition reduced the percent-correct responses compared to the static stimulus, suggesting that this manipulation affected the detectability of the sign of the accommodative stimulus. Several possible reasons and implications of this result are discussed.