Chitin nanofibrils suppress skin inflammation in atopic dermatitis-like skin lesions in NC/Nga mice

Ryotaro Izumi; Kazuo Azuma; Hironori Izawa; Minoru Morimoto; Masaaki Nagashima; Tomohiro Osaki; Takeshi Tsuka; Tomohiro Imagawa; Norihiko Ito; Yoshiharu Okamoto; Hiroyuki Saimoto; Shinsuke Ifuku

doi:10.1016/j.carbpol.2016.03.068

Chitin nanofibrils suppress skin inflammation in atopic dermatitis-like skin lesions in NC/Nga mice

Carbohydr Polym. 2016 Aug 1:146:320-7. doi: 10.1016/j.carbpol.2016.03.068. Epub 2016 Mar 25.

Authors

Affiliations

¹ Graduate School of Engineering, Tottori University, Tottori 680-8533, Japan. Electronic address: jastam0ment@gmail.com.
² Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8533, Japan. Electronic address: kazu-azuma@muses.tottori-u.ac.jp.
³ Graduate School of Engineering, Tottori University, Tottori 680-8533, Japan. Electronic address: h-izawa@chem.tottori-u.ac.jp.
⁴ Division of Instrumental Analysis, Research Center for Bioscience and Technology, Tottori University, Tottori 680-8550, Japan. Electronic address: morimoro@chem.tottori-u.ac.jp.
⁵ Regional Industrial Academic Cooperation, Tottori University, Tottori 680-0833, Japan. Electronic address: nagashima@cjrd.tottori-u.ac.jp.
⁶ Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8533, Japan. Electronic address: tosaki@muses.tottori-u.ac.jp.
⁷ Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8533, Japan. Electronic address: tsuka@muses.tottori-u.ac.jp.
⁸ Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8533, Japan. Electronic address: imagawat@muses.tottori-u.ac.jp.
⁹ Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8533, Japan. Electronic address: taro@muses.tottori-u.ac.jp.
¹⁰ Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8533, Japan. Electronic address: yokamoto@muses.tottori-u.ac.jp.
¹¹ Graduate School of Engineering, Tottori University, Tottori 680-8533, Japan. Electronic address: saimoto@chem.tottori-u.ac.jp.
¹² Graduate School of Engineering, Tottori University, Tottori 680-8533, Japan. Electronic address: sifuku@chem.tottori-u.ac.jp.

PMID: 27112880
DOI: 10.1016/j.carbpol.2016.03.068

Abstract

We evaluated the effect of chitin nanofibril (CNF) application via skin swabs on an experimental atopic dermatitis (AD) model. AD scores were lower, and hypertrophy and hyperkeratosis of the epidermis were suppressed after CNF treatment. Furthermore, inflammatory cell infiltration in both the epidermis and dermis was inhibited. CNFs also attenuated histological scores. The suppressive effects of CNFs were equal to those of corticosteroid application; however, chitin did not show these effects. CNF application might have anti-infllammatory effects via suppression of the activation of nuclear factor-kappa B, cyclooxygenase-2, and inducible nitric oxide synthase. In an early-stage model of experimental AD, CNFs suppressed AD progression to the same extent as corticosteroids. They also suppressed skin inflammation and IgE serum levels. Our findings indicate that CNF application could aid in the prevention or treatment of AD skin lesions.

Keywords: Atopic dermatitis; Chitin nanofibril; Corticosteroids; Cyclooxygenase-2; Inducible nitric oxide synthase; Nuclear factor-kappa B.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chitin / chemistry
Chitin / pharmacology
Chitin / therapeutic use*
Dermatitis, Atopic / therapy*
Inflammation / therapy
Mice
Nanofibers*
Skin / drug effects

Substances

Chitin