Culture medium pH influence on Gluconacetobacter physiology: Cellulose production rate and yield enhancement in presence of multiple carbon sources

Fatima Yassine; Nathalie Bassil; Roula Flouty; Ali Chokr; Antoine El Samrani; Gisèle Boiteux; Mario El Tahchi

doi:10.1016/j.carbpol.2016.02.003

Culture medium pH influence on Gluconacetobacter physiology: Cellulose production rate and yield enhancement in presence of multiple carbon sources

Carbohydr Polym. 2016 Aug 1:146:282-91. doi: 10.1016/j.carbpol.2016.02.003. Epub 2016 Feb 17.

Authors

Fatima Yassine¹, Nathalie Bassil², Roula Flouty², Ali Chokr³, Antoine El Samrani⁴, Gisèle Boiteux⁵, Mario El Tahchi⁶

Affiliations

¹ LBMI, Department of Physics, Lebanese University, Faculty of Sciences II, PO Box 90656, Jdeidet, Lebanon; PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies, Lebanese University, PO Box 5, Hadat, Lebanon; Lyon University, Lyon1 University, IMP@LYON1, UMR CNRS 5223 «Ingénierie des Matériaux Polymères», Villeurbanne, France; Laboratory of Microbiology, Department of Biology, Lebanese University, Faculty of Sciences I, PO Box 5, Hadat, Lebanon. Electronic address: fatima.yassine89@yahoo.com.
² LBMI, Department of Physics, Lebanese University, Faculty of Sciences II, PO Box 90656, Jdeidet, Lebanon.
³ PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies, Lebanese University, PO Box 5, Hadat, Lebanon; Laboratory of Microbiology, Department of Biology, Lebanese University, Faculty of Sciences I, PO Box 5, Hadat, Lebanon.
⁴ PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies, Lebanese University, PO Box 5, Hadat, Lebanon.
⁵ Lyon University, Lyon1 University, IMP@LYON1, UMR CNRS 5223 «Ingénierie des Matériaux Polymères», Villeurbanne, France.
⁶ LBMI, Department of Physics, Lebanese University, Faculty of Sciences II, PO Box 90656, Jdeidet, Lebanon; PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies, Lebanese University, PO Box 5, Hadat, Lebanon.

PMID: 27112876
DOI: 10.1016/j.carbpol.2016.02.003

Abstract

Gluconacetobacter genera are valued for bacterial cellulose (BC) and acetic acid production. BC is produced at optimal yields in classical microbiological media that are expensive for a large scale of production. In addition, BC usage for industrial purposes is limited due to low conversion rate into cellulose and to long incubation duration. In this paper, Gluconacetobacter isolated from apple vinegar was kinetically studied to evaluate cellulose production in presence of different carbon sources. Acetic and citric acid effect on Gluconacetobacter metabolism is clarified. It was shown that Gluconacetobacter uses glucose as a primary carbon source for cells growth and products formation. Acetic acid employment as a co-carbon source in Hestrin Schramm medium showed an increase of 17% in BC yield with a moderate decrease in the crystallite size of the resulting polymer.

Keywords: Acetic acid; Carbon source; Cellulosic biofilm; Conversion rate; Gluconacetobacter; pH.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbon / metabolism*
Cellulose / biosynthesis*
Culture Media / chemistry*
Culture Media / metabolism*
Gluconacetobacter / physiology*
Hydrogen-Ion Concentration
Industrial Microbiology*

Substances

Culture Media
Carbon
Cellulose