An Overview of Methods in Plant Nitric Oxide (NO) Research: Why Do We Always Need to Use Multiple Methods?

Hideo Yamasaki; Naoko S Watanabe; Yasuko Sakihama; Michael F Cohen

doi:10.1007/978-1-4939-3600-7_1

An Overview of Methods in Plant Nitric Oxide (NO) Research: Why Do We Always Need to Use Multiple Methods?

Methods Mol Biol. 2016:1424:1-14. doi: 10.1007/978-1-4939-3600-7_1.

Authors

Hideo Yamasaki¹, Naoko S Watanabe², Yasuko Sakihama³, Michael F Cohen^{4

5}

Affiliations

¹ Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, 903-0213, Japan. yamasaki@sci.u-ryukyu.ac.jp.
² Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, 903-0213, Japan.
³ Research Faculty of Agriculture, Hokkaido University, Kita Ku, Kita 9, Nishi 9, Sapporo, Hokkaido, 060-8589, Japan.
⁴ Department of Biology, Sonoma State University, Rohnert Park, CA, 94928, USA.
⁵ Biological Systems Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna-son, Kunigami-gun, Okinawa, 904-0495, Japan.

PMID: 27094406
DOI: 10.1007/978-1-4939-3600-7_1

Abstract

The free radical nitric oxide (NO) is a universal signaling molecule among living organisms. To investigate versatile functions of NO in plants it is essential to analyze biologically produced NO with an appropriate method. Owing to the uniqueness of NO, plant researchers may encounter difficulties in applying methods that have been developed for mammalian study. Based on our experience, we present here a practical guide to NO measurement fitted to plant biology.

Keywords: Chemiluminescence detection; DAF; Electrochemical detection; Nitric oxide; RNS; ROS; RSS; cPTIO.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Luminescence
Nitric Oxide / metabolism*
Plants / metabolism*

Substances

Nitric Oxide