Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis

Fuqiang Huang; Zhisheng Dang; Yutaka Suzuki; Terumi Horiuchi; Kinpei Yagi; Hirokazu Kouguchi; Takao Irie; Kyeongsoon Kim; Yuzaburo Oku

doi:10.1371/journal.pntd.0004634

Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis

PLoS Negl Trop Dis. 2016 Apr 19;10(4):e0004634. doi: 10.1371/journal.pntd.0004634. eCollection 2016 Apr.

Authors

Fuqiang Huang^{1

2}, Zhisheng Dang³, Yutaka Suzuki⁴, Terumi Horiuchi⁴, Kinpei Yagi⁵, Hirokazu Kouguchi⁵, Takao Irie⁵, Kyeongsoon Kim¹, Yuzaburo Oku¹

Affiliations

¹ Parasitology Laboratory, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
² The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
³ National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai, China.
⁴ Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan.
⁵ Department of Infectious Disease, Hokkaido Institute of Public Health, Sapporo, Hokkaido, Japan.

Abstract

Alveolar echinococcosis is a worldwide zoonosis of great public health concern. Analysis of genome data for Echinococcus multilocularis has identified antigen families that can be used in diagnostic assays and vaccine development. However, little gene expression data is available for antigens of the egg and early larval stages. To address this information gap, we used a Next-Generation Sequencing approach to investigate three different stages (non-activated and activated oncospheres, and early stage metacestodes) of E. multilocularis (Nemuro strain). Transcriptome data analysis revealed that some diagnostic antigen gp50 isoforms and the antigen Eg95 family dominated in activated oncospheres, and the antigen B family dominated in early stage metacestodes. Furthermore, heat shock proteins and antigen II/3 are constantly expressed in the three stages. The expression pattern of various known antigens in E. multilocularis may give fundamental information for choosing candidate genes used in diagnosis and vaccine development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, Helminth / biosynthesis
Echinococcus multilocularis / genetics*
Echinococcus multilocularis / growth & development*
High-Throughput Nucleotide Sequencing
Transcriptome*

Substances

Antigens, Helminth

Grants and funding

This work was supported by JSPS KAKENHI (No. 25450425). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.