Facile method to prepare silk fibroin/hyaluronic acid films for vascular endothelial growth factor release

Carbohydr Polym. 2016 Jun 5:143:301-9. doi: 10.1016/j.carbpol.2016.01.023. Epub 2016 Jan 12.

Abstract

A facile approach was proposed to prepare silk fibroin (SF) and hyaluronic acid (HA) composite films from aqueous solution without crosslinking or any post treatment. Only by controlling the HA content and film formation temperature during the film casting, the HA/SF films with different composition were prepared. The films were then characterized by structural characteristics, thermal stability, morphology, water stability, water absorption, mechanical properties. After immersing in water for 24h, all of the films showed good structural integrity. The degradation rate of the HA/SF films in protease XIV can be controlled by changing the film formation temperature and HA content. Decreasing the temperature and adding HA resulted in the rapid release of VEGF (vascular endothelial growth factor) from the HA/SF films. Overall, the 5% HA/SF films formed at 37°C with more rapid VEGF release exhibited great potential in drug delivery, especially when the rapid vascularization was needed.

Keywords: Facile method; Film; Hyaluronic acid; Silk fibroin; VEGF release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bombyx
  • Drug Carriers / chemistry*
  • Drug Liberation
  • Fibroins / chemistry*
  • Fibroins / isolation & purification
  • Humans
  • Hyaluronic Acid / chemistry*
  • Porosity
  • Pronase / chemistry
  • Spectroscopy, Fourier Transform Infrared
  • Surface Properties
  • Temperature
  • Tensile Strength
  • Vascular Endothelial Growth Factor A / chemistry*
  • Water / chemistry
  • X-Ray Diffraction

Substances

  • Drug Carriers
  • Vascular Endothelial Growth Factor A
  • fibroin, silkworm
  • Water
  • Hyaluronic Acid
  • Fibroins
  • Pronase