Characterization and application of chondroitin sulfate/polyvinyl alcohol nanofibres prepared by electrospinning

Carbohydr Polym. 2016 Jun 5:143:239-45. doi: 10.1016/j.carbpol.2016.02.013. Epub 2016 Feb 4.

Abstract

Composite nanofibres were prepared by electrospinning from a solution of chondroitin sulfate and polyvinyl alcohol. The chondroitin sulfate/polyvinyl alcohol (CS/PVA) mass ratios of 7/3 has a uniform and smooth morphology, and the average diameter of the nanofibres was 136nm. Combretastatin A-4 phosphate was loaded on the nanofibres and used as a model for testing drug release from the nanofibres crosslinked with glutaric dialdehyde. The morphology and structure of the nanofibres was determined using scanning electron microscopy. In order to assess their possible application to tissue engineering scaffolds, the toxicity and cytocompatibility of the nanofibres were tested by methylthiazolydiphenyl-tetrazolium bromide assay.

Keywords: Chondroitin sulfate; Electrospinning; Nanofibres.

MeSH terms

  • Animals
  • Cell Line
  • Chondroitin Sulfates / chemistry*
  • Chondroitin Sulfates / toxicity
  • Cross-Linking Reagents / chemistry
  • Diffusion
  • Electrochemical Techniques
  • Fluorescence
  • Glutaral / chemistry
  • Mice
  • Microscopy, Electron, Scanning
  • Nanofibers / chemistry*
  • Nanofibers / toxicity
  • Polyvinyl Alcohol / chemistry*
  • Polyvinyl Alcohol / toxicity
  • Stilbenes / chemistry
  • Surface Tension

Substances

  • Cross-Linking Reagents
  • Stilbenes
  • Polyvinyl Alcohol
  • Chondroitin Sulfates
  • fosbretabulin
  • Glutaral