Orderly nucleic acid aggregates by electrostatic self-assembly in single cells for miRNA detection and visualizing

Yuanyuan Wang; Zhaopeng Yu; Zhen Zhang; Rui Ren; Shusheng Zhang

doi:10.1039/c6an00160b

Orderly nucleic acid aggregates by electrostatic self-assembly in single cells for miRNA detection and visualizing

Analyst. 2016 May 10;141(10):2861-4. doi: 10.1039/c6an00160b.

Authors

Yuanyuan Wang¹, Zhaopeng Yu², Zhen Zhang², Rui Ren³, Shusheng Zhang²

Affiliations

¹ Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan 250014, China.
² Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China. zhangzhen19801981@126.com.
³ Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan 250014, China and Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China. zhangzhen19801981@126.com.

PMID: 27063644
DOI: 10.1039/c6an00160b

Abstract

Orderly nucleic acid aggregates (ONAAs) self-assembled on mesoporous silica nanoparticles (MSNs) with positively charged aminopropyl groups (PC) were firstly developed. Interestingly, a novel electrostatic DNA self-assembly could realize hybridization chain reaction (HCR) on the surface of PCMSNs in single cells. Significantly, a non-destructive amplification strategy based on ONAAs-PCMSNs was successfully developed for miRNA detection and in situ imaging by the prominent and agminated fluorescence-bright spots in living cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
DNA
Humans
MicroRNAs / analysis*
Nanoparticles
Nucleic Acid Hybridization
Nucleic Acids / chemistry*
Single-Cell Analysis*
Static Electricity*

Substances

MicroRNAs
Nucleic Acids
DNA