Quantification of the environmental neurotoxin annonacin in Rat brain by UPLC-MS/MS

Natacha Bonneau; Isabelle Schmitz-Afonso; Alain Brunelle; David Touboul; Pierre Champy

doi:10.1016/j.toxicon.2016.03.015

Quantification of the environmental neurotoxin annonacin in Rat brain by UPLC-MS/MS

Toxicon. 2016 Aug:118:129-33. doi: 10.1016/j.toxicon.2016.03.015. Epub 2016 Apr 4.

Authors

Natacha Bonneau¹, Isabelle Schmitz-Afonso², Alain Brunelle², David Touboul², Pierre Champy³

Affiliations

¹ Laboratoire de Pharmacognosie, BioCIS, Univ. Paris-Sud, CNRS, Université Paris-Saclay, UFR Pharmacie, 5 rue J.-B. Clément, 92290, Châtenay-Malabry, France.
² Institut de Chimie des Substances Naturelles, CNRS UPR2301, Université Paris-Saclay, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
³ Laboratoire de Pharmacognosie, BioCIS, Univ. Paris-Sud, CNRS, Université Paris-Saclay, UFR Pharmacie, 5 rue J.-B. Clément, 92290, Châtenay-Malabry, France. Electronic address: pierre.champy@u-psud.fr.

PMID: 27058514
DOI: 10.1016/j.toxicon.2016.03.015

Abstract

The Annonaceous acetogenin annonacin is an environmental neurotoxin identified in the pulp of several fruits of the Annonaceae family, whose consumption was linked to the occurrence of sporadic atypical Parkinsonism with dementia. A method for its quantification in Rat brain homogenates by UPLC-MS/MS in selected reaction monitoring (SRM) mode was developed and validated. This method was applied to the quantitation of annonacin in Rat brain after intravenous (0.5 mg/kg) and oral (10 mg/kg, 100 mg/kg) administration.

Keywords: Acetogenin; Annonaceae; Annonacin; Distribution; Rat brain; UPLC-MS/MS.

Publication types

Validation Study

MeSH terms

Administration, Oral
Animals
Biotransformation
Blood-Brain Barrier / drug effects
Blood-Brain Barrier / metabolism
Brain / metabolism*
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Electron Transport Complex I / antagonists & inhibitors
Electron Transport Complex I / metabolism
Environmental Pollutants / administration & dosage
Environmental Pollutants / analysis
Environmental Pollutants / toxicity*
Enzyme Inhibitors / administration & dosage
Enzyme Inhibitors / analysis
Enzyme Inhibitors / toxicity
Furans / administration & dosage
Furans / analysis
Furans / toxicity*
Injections, Intravenous
Lactones / administration & dosage
Lactones / analysis
Lactones / toxicity*
Male
Neurons / chemistry
Neurons / metabolism*
Rats, Wistar
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Tissue Distribution
Toxicokinetics
Toxins, Biological / administration & dosage
Toxins, Biological / analysis
Toxins, Biological / toxicity*

Substances

Environmental Pollutants
Enzyme Inhibitors
Furans
Lactones
Toxins, Biological
annonacin
Electron Transport Complex I