The structure of O-polysaccharides isolated from plant pathogenic bacteria Pectobacterium wasabiae IFB5408 and IFB5427

Karolina Ossowska; Małgorzata Czerwicka; Wojciech Sledz; Sabina Zoledowska; Agata Motyka; Sylwia Szulta; Ewa Lojkowska; Zbigniew Kaczyński

doi:10.1016/j.carres.2016.03.017

The structure of O-polysaccharides isolated from plant pathogenic bacteria Pectobacterium wasabiae IFB5408 and IFB5427

Carbohydr Res. 2016 May 13:426:46-9. doi: 10.1016/j.carres.2016.03.017. Epub 2016 Mar 22.

Authors

Karolina Ossowska¹, Małgorzata Czerwicka¹, Wojciech Sledz², Sabina Zoledowska², Agata Motyka², Sylwia Szulta¹, Ewa Lojkowska², Zbigniew Kaczyński³

Affiliations

¹ Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland.
² Department of Biotechnology, Intercollegiate Faculty of Biotechnology University of Gdansk & Medical University of Gdansk, Abrahama 58, 80-307 Gdansk, Poland.
³ Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland. Electronic address: zbigniew.kaczynski@ug.edu.pl.

PMID: 27058296
DOI: 10.1016/j.carres.2016.03.017

Abstract

O-Polysaccharides were isolated from the lipopolysaccharides of two strains of plant pathogenic bacteria Pectobacterium wasabiae isolated in Poland in 2013 (IFB5408 and IFB5427). The purified polysaccharides were analyzed using 1D and 2D NMR spectroscopy ((1)H, DQF-COSY, TOCSY, ROESY, HSQC, HSQC-TOCSY, and HMBC) and the chemical methods. Sugar and methylation analyses of native polysaccharides, absolute configuration assignment of constituent monosaccharides together with NMR spectroscopy data revealed that the chemical structures of both O-polysaccharides are the same.

Keywords: NMR; O-polysaccharide; Pectobacterium wasabiae; Structure.

MeSH terms

Carbohydrate Conformation
Magnetic Resonance Spectroscopy
Pectobacterium / chemistry*
Plant Diseases / microbiology*
Polysaccharides / chemistry*
Polysaccharides / isolation & purification*
Reference Standards

Substances

Polysaccharides