Biosensing made easy with PEG-targeted bi-specific antibodies

Lyndon J Raftery; Yadveer S Grewal; Christopher B Howard; Martina L Jones; Muhammad J A Shiddiky; Laura G Carrascosa; Kristofer J Thurecht; Stephen M Mahler; Matt Trau

doi:10.1039/c6cc01916a

Biosensing made easy with PEG-targeted bi-specific antibodies

Chem Commun (Camb). 2016 Apr 28;52(33):5730-3. doi: 10.1039/c6cc01916a.

Authors

Lyndon J Raftery¹, Yadveer S Grewal², Christopher B Howard³, Martina L Jones¹, Muhammad J A Shiddiky², Laura G Carrascosa², Kristofer J Thurecht⁴, Stephen M Mahler⁵, Matt Trau⁶

Affiliations

¹ Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD 4072, Australia.
² Centre for Personalised NanoMedicine, AIBN, University of Queensland, Brisbane, QLD 4072, Australia.
³ Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD 4072, Australia and Centre for Advanced Imaging (CAI), University of Queensland, Brisbane, QLD 4072, Australia.
⁴ Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD 4072, Australia and Centre for Advanced Imaging (CAI), University of Queensland, Brisbane, QLD 4072, Australia and ARC Centre of Excellence in Convergent BioNano Science and Technology, University of Queensland, Brisbane, QLD 4072, Australia.
⁵ Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD 4072, Australia and School of Chemical Engineering, University of Queensland, Brisbane, QLD 4072, Australia. s.mahler.eng.uq.edu.au.
⁶ Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD 4072, Australia and Centre for Personalised NanoMedicine, AIBN, University of Queensland, Brisbane, QLD 4072, Australia and School of Chemistry and Molecular Biosciences (SCMB), University of Queensland, Brisbane, QLD 4072, Australia. m.trau@uq.edu.au.

PMID: 27045479
DOI: 10.1039/c6cc01916a

Abstract

Whilst recent advances in nanotechnology have yielded many new biosensing capabilities, innovative biological attachment and detection modalities remain relatively underdeveloped. Bi-specific antibodies (bsAbs)--which exhibit binding capability for two separate targets--offer an inherent advantage over conventional antibody reagents by significantly simplifying sensor surface preparation. Herein, we report the deployment of bsAbs for simultaneous attachment to a polymer-coated transducer and label-free, electrochemical (EC) detection of target antigens.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Bispecific / metabolism*
Biosensing Techniques*
Polyethylene Glycols / metabolism*

Substances

Antibodies, Bispecific
Polyethylene Glycols