Reproduction of post-weaning multi-systemic wasting syndrome in an animal disease model as a tool for vaccine testing under controlled conditions

Res Vet Sci. 2016 Apr:105:143-52. doi: 10.1016/j.rvsc.2016.01.004. Epub 2016 Feb 4.

Abstract

Snatch farrowed, colostrum deprived piglets were inoculated with different combinations of porcine circovirus 2, porcine parvovirus and Erysipelothrix rhusiopathiae candidate vaccines. 10 piglets were mock-vaccinated. Following virus challenge with a combined porcine circovirus 2/porcine parvovirus inoculum, all animals were monitored and samples taken for serology, immunohistochemistry and qPCR. At 24 dpc all non-vaccinated animals remaining were exhibiting signs of post-weaning multi-systemic wasting syndrome which was confirmed by laboratory analysis. Details of the study, analysis of samples and performance of the candidate vaccines are described.

Keywords: Animal model; Porcine circovirus; Porcine parvovirus; Quantitative PCR; Vaccine.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Vaccines / pharmacology
  • Circovirus / immunology*
  • Disease Models, Animal
  • Erysipelothrix / immunology
  • Erysipelothrix Infections / immunology
  • Erysipelothrix Infections / microbiology
  • Parvoviridae Infections / immunology
  • Parvoviridae Infections / virology
  • Parvovirus, Porcine / immunology
  • Porcine Postweaning Multisystemic Wasting Syndrome / immunology*
  • Porcine Postweaning Multisystemic Wasting Syndrome / virology
  • Swine
  • Swine Diseases / immunology
  • Swine Diseases / microbiology
  • Swine Diseases / virology
  • Viral Vaccines / pharmacology*

Substances

  • Bacterial Vaccines
  • Viral Vaccines