Abstract
Selective plane illumination microscopy can image biological samples at a high spatiotemporal resolution. Complex sample preparation and system alignment normally limit the throughput of the method. Using femtosecond laser micromachining, we created an integrated optofluidic device that allows obtaining continuous flow imaging, three-dimensional reconstruction and high-throughput analysis of large multicellular spheroids at a subcellular resolution.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Equipment Design
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High-Throughput Screening Assays / instrumentation*
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Histones / chemistry
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Histones / genetics
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Histones / metabolism
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Humans
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Imaging, Three-Dimensional / instrumentation*
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Lab-On-A-Chip Devices*
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Luminescent Proteins / chemistry
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Microscopy, Fluorescence / instrumentation
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Microscopy, Fluorescence / methods*
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Nanoparticles / chemistry
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Recombinant Fusion Proteins / metabolism
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Red Fluorescent Protein
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Single-Cell Analysis / instrumentation
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Spheroids, Cellular / cytology
Substances
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Histones
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Luminescent Proteins
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Recombinant Fusion Proteins