Rapid Flow Cytometry Test for Identification of Different Carbapenemases in Enterobacteriaceae

Ana P Silva; Isabel Faria-Ramos; Elisabete Ricardo; Isabel M Miranda; Maria J Espinar; Sofia Costa-de-Oliveira; Rafael Cantón; Acácio G Rodrigues; Cidália Pina-Vaz

doi:10.1128/AAC.02947-15

Rapid Flow Cytometry Test for Identification of Different Carbapenemases in Enterobacteriaceae

Antimicrob Agents Chemother. 2016 May 23;60(6):3824-6. doi: 10.1128/AAC.02947-15. Print 2016 Jun.

Authors

Ana P Silva¹, Isabel Faria-Ramos², Elisabete Ricardo², Isabel M Miranda², Maria J Espinar³, Sofia Costa-de-Oliveira², Rafael Cantón⁴, Acácio G Rodrigues², Cidália Pina-Vaz³

Affiliations

¹ Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal atsilva@med.up.pt.
² Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal.
³ Microbiology Department, Faculty of Medicine, University of Porto, Porto, Portugal Center for Research in Health Technologies and Information Systems (CINTESIS), Porto, Portugal Microbiology Department, Centro Hospitalar São João, Porto, Portugal.
⁴ Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.

Abstract

A flow cytometry test was developed to identify carbapenemase production by Enterobacteriaceae and to discriminate between the different types of carbapenemases (classes A, B, and D). It is based on the detection of meropenem activity against bacteria, coupled with different carbapenemase inhibitors, which is assessed by flow cytometry. It represents a convenient, fast, and reliable approach (100% sensitivity and 100% specificity) for the detection and characterization of different carbapenemases.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / antagonists & inhibitors
Bacterial Proteins / classification*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Boronic Acids / pharmacology
Cloxacillin / pharmacology
Edetic Acid / pharmacology
Enterobacteriaceae / drug effects*
Enterobacteriaceae / enzymology
Enterobacteriaceae / genetics
Enterobacteriaceae / growth & development
Enterobacteriaceae Infections / microbiology
Enzyme Inhibitors / pharmacology*
Flow Cytometry / methods*
Gene Expression
Humans
Meropenem
Penicillins / pharmacology
Thienamycins / pharmacology*
beta-Lactamases / classification*
beta-Lactamases / genetics
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
Bacterial Proteins
Boronic Acids
Enzyme Inhibitors
Penicillins
Thienamycins
temocillin
3-aminobenzeneboronic acid
Edetic Acid
beta-Lactamases
carbapenemase
Meropenem
Cloxacillin