Detection of Antileukocytic Antibodies in Blood Serum using Lymphocytes and Latex Microspheres Carrying HLA-Antigens upon Alloimmunization of Women with Recurrent Pregnancy Loss

Bull Exp Biol Med. 2016 Mar;160(5):722-6. doi: 10.1007/s10517-016-3260-2. Epub 2016 Mar 29.

Abstract

Anti-HLA-antibodies were detected using cross-reaction of blood serum with allogenic T and B cells and latex microspheres coated with HLA-I and HLA-II antigens. HLA+ and HLA-sera obtained from women before and after allogeneic immunization were tested. The results obtained by these methods significantly differed. The test with latex microspheres detected antibodies to HLA-I and HLA-II antigens with high sensitivity and specificity and can be used for assessment of clinical significance of alloantibody detection when using alloimmunization in the therapy of gestation disorders.

Keywords: HLA; alloimmunization; antibodies to human leukocyte antigens; flow cytometry; recurrent abortion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abortion, Habitual / immunology*
  • Antibodies / blood*
  • Antibodies / immunology*
  • B-Lymphocytes / immunology
  • Female
  • Histocompatibility Antigens Class I / immunology*
  • Histocompatibility Antigens Class II / immunology*
  • Humans
  • Leukocytes / immunology*
  • Microspheres
  • Pregnancy
  • Sensitivity and Specificity
  • T-Lymphocytes / immunology

Substances

  • Antibodies
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II