Lipase cocktail for efficient conversion of oils containing phospholipids to biodiesel

Jerome Amoah; Shih-Hsin Ho; Shinji Hama; Ayumi Yoshida; Akihito Nakanishi; Tomohisa Hasunuma; Chiaki Ogino; Akihiko Kondo

doi:10.1016/j.biortech.2016.03.062

Lipase cocktail for efficient conversion of oils containing phospholipids to biodiesel

Bioresour Technol. 2016 Jul:211:224-30. doi: 10.1016/j.biortech.2016.03.062. Epub 2016 Mar 14.

Authors

Jerome Amoah¹, Shih-Hsin Ho², Shinji Hama³, Ayumi Yoshida³, Akihito Nakanishi², Tomohisa Hasunuma², Chiaki Ogino¹, Akihiko Kondo⁴

Affiliations

¹ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan.
² Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan.
³ Bio-energy Corporation, Research and Development Laboratory, 2-9-7 Minaminanamatsu, Amagasaki 660-0053, Japan.
⁴ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan. Electronic address: akondo@kobe-u.ac.jp.

PMID: 27019125
DOI: 10.1016/j.biortech.2016.03.062

Abstract

The presence of phospholipid has been a challenge in liquid enzymatic biodiesel production. Among six lipases that were screened, lipase AY had the highest hydrolysis activity and a competitive transesterification activity. However, it yielded only 21.1% FAME from oil containing phospholipids. By replacing portions of these lipases with a more robust bioFAME lipase, CalT, the combination of lipase AY-CalT gave the highest FAME yield with the least amounts of free fatty acids and partial glycerides. A higher methanol addition rate reduced FAME yields for lipase DF-CalT and A10D-CalT combinations while that of lipase AY-CalT combination improved. Optimizing the methanol addition rate for lipase AY-CalT resulted in a FAME yield of 88.1% at 2h and more than 95% at 6h. This effective use of lipases could be applied for the rapid and economic conversion of unrefined oils to biodiesel.

Keywords: Biodiesel; Enzyme biocatalysis; Mixed lipase; Phospholipid; Transesterification.

MeSH terms

Biofuels*
Hydrolysis
Lipase / chemistry*
Oils / chemistry*
Phospholipids / chemistry*

Substances

Biofuels
Oils
Phospholipids
Lipase