A Novel Conductive Poly(3,4-ethylenedioxythiophene)-BSA Film for the Construction of a Durable HRP Biosensor Modified with NanoAu Particles

Sensors (Basel). 2016 Mar 15;16(3):374. doi: 10.3390/s16030374.

Abstract

In this study, we have investigated the contribution of bovine serum albumin (BSA) to the durability of the electrochemically synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) film on a platinum (Pt) electrode. The electrode was capable to effectively adsorb the nano Au particles (AuNPs) to form a uniform layout, which was then able to immobilize the horseradish peroxidase (HRP) to construct a functional HRP/AuNPs/PEDOT(BSA)/Pt biosensor. Cyclic voltammetry was employed to evaluate the performance of the biosensor through the measurement of hydrogen peroxide. Our results revealed a satisfied linear correlation between the cathodic current and the concentration of H2O2. Furthermore, the addition of oxidized form of nicotinamide adenine dinucleotide, or NAD⁺, as the electron transfer mediator in the detection solution could dramatically enhance the sensitivity of detection by about 35.5%. The main advantages of the current biosensor are its durability, sensitivity, reliability, and biocompatibility.

Keywords: HRP biosensor; bovine serum albumin; electron transfer mediator; nano Au particle; poly(3,4-ethylenedioxythiophene).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biosensing Techniques / methods*
  • Bridged Bicyclo Compounds, Heterocyclic / chemistry
  • Cattle
  • Electrodes
  • Enzymes, Immobilized / chemistry
  • Gold / chemistry
  • Horseradish Peroxidase / chemistry
  • Hydrogen Peroxide / isolation & purification*
  • Nanoparticles / chemistry*
  • Polymers / chemistry
  • Serum Albumin, Bovine / chemistry*

Substances

  • Bridged Bicyclo Compounds, Heterocyclic
  • Enzymes, Immobilized
  • Polymers
  • poly(3,4-ethylene dioxythiophene)
  • Serum Albumin, Bovine
  • Gold
  • Hydrogen Peroxide
  • Horseradish Peroxidase