Identification of the Bcl-2 family protein gene BOK from orange-spotted grouper (Epinephelus coioides) involved in SGIV infection

Fish Shellfish Immunol. 2016 May:52:9-15. doi: 10.1016/j.fsi.2016.03.026. Epub 2016 Mar 16.

Abstract

Apoptosis plays vital roles in many physiological process and immune response. BOK is one of the central regulators in apoptosis. In this study, a new BOK homolog (Ec-BOK) was cloned and characterized from Orange-spotted grouper, Epinephelus coioides. Ec-BOK encoded a 210 amino acid peptides which shared 97% identity to Stegastes partitus BOK protein, contained four BH domains and one transmembrane region. Ec-BOK widely expressed in all analyzed tissues with the higher expressions in kidney and spleen. Its expression level was up-regulated after SGIV infection in vitro. Further analysis revealed that overexpression of Ec-BOK inhibited viral genes transcriptions and virus replication in fish cell. Our findings suggested that Ec-BOK might play a role in the immune response against virus.

Keywords: BOK; Epinephelus coioides; Singapore grouper iridovirus (SGIV); Viral gene; Viral replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bass / classification
  • Bass / genetics*
  • Bass / immunology
  • Bass / metabolism
  • Cloning, Molecular
  • DNA Virus Infections / immunology
  • DNA Virus Infections / veterinary*
  • DNA Virus Infections / virology
  • DNA, Complementary / genetics
  • DNA, Complementary / metabolism
  • Fish Diseases / immunology*
  • Fish Diseases / virology
  • Fish Proteins / chemistry
  • Fish Proteins / genetics*
  • Fish Proteins / metabolism
  • Organ Specificity
  • Phylogeny
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Ranavirus / physiology*
  • Sequence Alignment / veterinary

Substances

  • DNA, Complementary
  • Fish Proteins
  • RNA, Messenger