Genetically humanized pigs exclusively expressing human insulin are generated through custom endonuclease-mediated seamless engineering

Yi Yang; Kepin Wang; Han Wu; Qin Jin; Degong Ruan; Zhen Ouyang; Bentian Zhao; Zhaoming Liu; Yu Zhao; Quanjun Zhang; Nana Fan; Qishuai Liu; Shimei Guo; Lei Bu; Yong Fan; Xiaofang Sun; Xiaoping Li; Liangxue Lai

doi:10.1093/jmcb/mjw008

Genetically humanized pigs exclusively expressing human insulin are generated through custom endonuclease-mediated seamless engineering

J Mol Cell Biol. 2016 Apr;8(2):174-7. doi: 10.1093/jmcb/mjw008. Epub 2016 Mar 18.

Authors

Yi Yang¹, Kepin Wang¹, Han Wu¹, Qin Jin¹, Degong Ruan¹, Zhen Ouyang¹, Bentian Zhao¹, Zhaoming Liu¹, Yu Zhao¹, Quanjun Zhang¹, Nana Fan¹, Qishuai Liu¹, Shimei Guo², Lei Bu³, Yong Fan⁴, Xiaofang Sun⁴, Xiaoping Li⁵, Liangxue Lai⁶

Affiliations

¹ Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
² Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
³ Leon H Charney Division of Cardiology, New York University School of Medicine, 522 First Avenue, New York, NY 10016, USA.
⁴ Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China.
⁵ Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China lai_liangxue@gibh.ac.cn li_xiaoping@gibh.ac.cn.
⁶ Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China lai_liangxue@gibh.ac.cn li_xiaoping@gibh.ac.cn.

PMID: 26993040
DOI: 10.1093/jmcb/mjw008

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Genetically Modified / genetics*
CRISPR-Associated Proteins / metabolism*
Female
Gene Editing / methods*
Humans
Insulin, Regular, Human / biosynthesis
Insulin, Regular, Human / genetics*
Insulin, Regular, Human / metabolism
Male
Swine / genetics*
Swine, Miniature / genetics
Transcription Activator-Like Effector Nucleases / metabolism*

Substances

CRISPR-Associated Proteins
Insulin, Regular, Human
Transcription Activator-Like Effector Nucleases