(1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1

Joanna R Watson; Daniel Nietlispach; Darerca Owen; Helen R Mott

doi:10.1007/s12104-016-9677-8

(1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1

Biomol NMR Assign. 2016 Oct;10(2):407-11. doi: 10.1007/s12104-016-9677-8. Epub 2016 Mar 18.

Authors

Joanna R Watson¹, Daniel Nietlispach¹, Darerca Owen¹, Helen R Mott²

Affiliations

¹ Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
² Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK. hrm28@bioc.cam.ac.uk.

Abstract

TOCA1 is a downstream effector protein of the small GTPase, Cdc42. It is a multi-domain protein that includes a membrane binding F-BAR domain, a homology region 1 (HR1) domain, which binds selectively to active Cdc42 and an SH3 domain. TOCA1 is involved in the regulation of actin dynamics in processes such as endocytosis, filopodia formation, neurite elongation, cell motility and invasion. Structural insight into the interaction between TOCA1 and Cdc42 will contribute to our understanding of the role of TOCA1 in actin dynamics. The (1)H, (15)N and (13)C NMR backbone and sidechain resonance assignment of the HR1 domain (12 kDa) presented here provides the foundation for structural studies of the domain and its interactions.

Keywords: Cdc42; Cytoskeleton; Endocytosis; GTPase; HR1; Signalling; TOCA1.

MeSH terms

Animals
Carrier Proteins / chemistry*
Carrier Proteins / metabolism*
Fatty Acid-Binding Proteins
Nuclear Magnetic Resonance, Biomolecular*
Protein Domains
Protein Structure, Secondary
Xenopus
Xenopus Proteins / chemistry*
Xenopus Proteins / metabolism*
cdc42 GTP-Binding Protein / metabolism*

Substances

Carrier Proteins
FNBP1L protein, Xenopus
Fatty Acid-Binding Proteins
Xenopus Proteins
cdc42 GTP-Binding Protein