Generation of integration-free induced pluripotent stem cells from a patient with Familial Mediterranean Fever (FMF)

Kerem Fidan; Gülnihal Kavaklıoğlu; Ayyub Ebrahimi; Can Özlü; Nur Zeynep Ay; Arzu Ruacan; Ahmet Gül; Tamer T Önder

doi:10.1016/j.scr.2015.10.018

Generation of integration-free induced pluripotent stem cells from a patient with Familial Mediterranean Fever (FMF)

Stem Cell Res. 2015 Nov;15(3):694-6. doi: 10.1016/j.scr.2015.10.018. Epub 2015 Nov 2.

Authors

Kerem Fidan¹, Gülnihal Kavaklıoğlu¹, Ayyub Ebrahimi¹, Can Özlü¹, Nur Zeynep Ay¹, Arzu Ruacan¹, Ahmet Gül², Tamer T Önder¹

Affiliations

¹ School of Medicine, Koc University, Istanbul 34450, Turkey.
² Faculty of Medicine, Istanbul University, Istanbul 34093, Turkey.

PMID: 26987928
DOI: 10.1016/j.scr.2015.10.018

Abstract

Fibroblasts from a Familial Mediterranean Fever (FMF) patient were reprogrammed with episomal vectors by using the Neon Transfection System for the generation of integration-free induced pluripotent stem cells (iPSCs). The resulting iPSC line was characterized to determine the expression of pluripotency markers, proper differentiation into three germ layers, the presence of normal chromosomal structures as well as the lack of genomic integration. A homozygous missense mutation in the MEFV gene (p.Met694Val), which lead to typical FMF phenotype, was shown to be present in the generated iPSC line.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Familial Mediterranean Fever / immunology*
Familial Mediterranean Fever / pathology
Fibroblasts / cytology
Fibroblasts / metabolism*
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*