Cross reactivity of S. aureus to murine cytokine assays: A source of discrepancy

Numan Javed; Guang Xue; Ailing Lu; Yue Xing; Yoichiro Iwakura; Hui Xiao; Hervé Lecoeur; Gerald F Späth; Guangxun Meng

doi:10.1016/j.cyto.2016.03.007

Cross reactivity of S. aureus to murine cytokine assays: A source of discrepancy

Cytokine. 2016 May:81:101-8. doi: 10.1016/j.cyto.2016.03.007. Epub 2016 Mar 12.

Authors

Numan Javed¹, Guang Xue², Ailing Lu², Yue Xing², Yoichiro Iwakura³, Hui Xiao², Hervé Lecoeur⁴, Gerald F Späth⁵, Guangxun Meng⁶

Affiliations

¹ Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China; Department of Microbiology & Molecular Genetics (MMG), Quaid-e-Azam Campus, University of the Punjab, Lahore, Pakistan.
² Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China.
³ Division of Experimental Animal Immunology, Center for Animal Disease Models, Research Institute for Biomedical Sciences, Tokyo University of Science, Chiba 278-0022, Japan.
⁴ Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China; Institut Pasteur, CNRS URA 2581, Unité de Parasitologie moléculaire et Signalisation, Paris, France.
⁵ Institut Pasteur, CNRS URA 2581, Unité de Parasitologie moléculaire et Signalisation, Paris, France.
⁶ Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China. Electronic address: gxmeng@ips.ac.cn.

PMID: 26978550
DOI: 10.1016/j.cyto.2016.03.007

Abstract

Staphylococcus aureus is one of the versatile Gram positive bacteria causing a range of diseases. Upon challenge, host immune cells recognize S. aureus and mount diverse immune responses including production of pro-inflammatory cytokines such as IL-1β and TNF-α. These cytokines are important mediators of inflammation which can be detected via various immunological methods such as enzyme linked immunosorbent assay (ELISA) and immunoblotting. In the current study, we found that a number of clinical isolates as well as laboratory strains of S. aureus exhibited cross reactivity with ELISA antibodies for murine IL-1β and TNF-α assays. This cross reactivity generates exaggerated false positive signals which can be a source of discrepancy for the understanding of real immune responses against S. aureus infection by host immune cells.

Keywords: Cross reactivity; IL-1β; Inflammasome; S. aureus; TNF-α.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blotting, Western
Cells, Cultured
Cross Reactions / immunology*
Cytokines / genetics
Cytokines / immunology*
Enzyme-Linked Immunosorbent Assay / methods*
Host-Pathogen Interactions / immunology
Interleukin-1beta / genetics
Interleukin-1beta / immunology
Macrophages / immunology*
Macrophages / metabolism
Macrophages / microbiology
Mice, Inbred C57BL
Mice, Knockout
Signal Transduction / genetics
Signal Transduction / immunology
Species Specificity
Staphylococcus aureus / classification
Staphylococcus aureus / genetics
Staphylococcus aureus / immunology*
Tumor Necrosis Factor-alpha / immunology

Substances

Cytokines
Interleukin-1beta
Tumor Necrosis Factor-alpha