Surface modification of bioresorbable polymer material (polycaprolactone, PCL) with abnormal glow discharge, initiated during radio-frequency magnetron sputtering of a hydroxyapatite target was investigated. Plasma treatment resulted in an increase of surface roughness of PCL, crystallite size, the surface free energy and hydrophilicity. Increased treatment time (30, 60, 150 seconds) provoked the polymer surface saturation with the sputtering target ions (calcium, phosphorus). The assessment of plasma exposure of PCL surface on bone marrow multipotent mesenchymal stromal cells behavior (BM MSCs) has been performed. Modification of the polymer surface with the abnormal glow discharge stimulated adhesion and subsequent proliferation of BM MSCs; thus, maximum values were achieved with the surface treatment for 60 s. This type of plasma modification did not affect cell viability (apoptosis, necrosis). Thus, the surface modification with abnormal glow discharge, initiated during radio-frequency magnetron sputtering of a hydroxyapatite target, appear to be a promising method of surface modification of bioresorbable polymer material (PCL) for tissue engineering.
V rabote izuchena vozmozhnost' modifitsirovaniia poverkhnosti biorezorbiruemogo polimernogo materiala (polikaprolaktona, PCL) v plazme anomal'no tleiushchego razriada, voznikaiushchego pri vysokochastotnom magnetronnom raspylenii tverdotel'noĭ misheni, izgotovlennoĭ iz gidroksiapatita. Issledovano izmenenie fiziko-khimicheskikh svoĭstv poverkhnosti polikaprolaktona v zavisimosti ot dlitel'nosti vozdeĭstviia plazmy (30, 60, 150 s). Plazmennaia obrabotka privela k uvelicheniiu sherokhovatosti i razmerov kristallitov na poverkhnosti PCL, povysheniiu poverkhnostnoĭ svobodnoĭ énergii i gidrofil'nosti. Uvelichenie vremeni obrabotki sposobstvovalo nasyshcheniiu poverkhnosti polimernogo materiala ionami raspyliaemoĭ misheni (kal'tsiem, fosforom). Provedena otsenka vliianiia dlitel'nosti ékspozitsii plazmennoĭ obrabotki PCL na povedenie kul'tiviruemykh na ikh poverkhnosti mul'tipotentnykh mezenkhimal'nykh stromal'nykh kletok kostnogo mozga (MMSK KM). Poverkhnost' polimera, modifitsirovannogo v plazme anomal'no tleiushchego razriada, stimulirovala adgeziiu i posleduiushchuiu proliferatsiiu MMSK KM. Pri étom maksimal'nye pokazateli dostigalis' pri obrabotke poverkhnosti v techenie 60 s. Dannyĭ vid plazmennoĭ obrabotki ne snizhal kletochnuiu zhiznesposobnost'. Takim obrazom, obrabotka v plazme anomal'no tleiushchego razriada, voznikaiushchego pri vysokochastotnom magnetronnom raspylenii tverdotel'noĭ misheni, izgotovlennoĭ iz gidroksiapatita, sposobstvuet povysheniiu biosovmestimosti poverkhnosti biodegradiruemykh polimernykh materialov i potentsial'no mozhet byt' ispol'zovana v tkanevoĭ inzhenerii.
Keywords: biocompatibility; free energy; hydrophilicity; polycaprolactone; roughness; surface modification.