Auricular vagus nerve stimulation promotes functional recovery and enhances the post-ischemic angiogenic response in an ischemia/reperfusion rat model

Ying Jiang; Longling Li; Jingxi Ma; Lina Zhang; Fei Niu; Tao Feng; Changqing Li

doi:10.1016/j.neuint.2016.02.009

Auricular vagus nerve stimulation promotes functional recovery and enhances the post-ischemic angiogenic response in an ischemia/reperfusion rat model

Neurochem Int. 2016 Jul:97:73-82. doi: 10.1016/j.neuint.2016.02.009. Epub 2016 Mar 8.

Authors

Ying Jiang¹, Longling Li², Jingxi Ma³, Lina Zhang³, Fei Niu⁴, Tao Feng⁵, Changqing Li⁶

Affiliations

¹ Department of Neurology, Center for Neurodegenerative Disease, Beijing Tiantan Hospital, Capital Medical University, #6 Tian Tan Xi Li Street, Beijing 100050, China; China National Clinical Research Center for Neurological Diseases, #6 Tian Tan Xi Li Street, Beijing 100050, China.
² Department of Neurology, Zhongshan Hospital, Xiamen University, #209 Hu Bin Nan Road, Xiamen, Fujian 361004, China.
³ Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, #76 Linjiang Road, Yuzhong District, Chongqing 400010, China.
⁴ Neurotrauma Laboratory, Beijing Neurosurgical Institute, Capital Medical University, #6 Tian Tan Xi Li Street, Beijing 100050, China.
⁵ Department of Neurology, Center for Neurodegenerative Disease, Beijing Tiantan Hospital, Capital Medical University, #6 Tian Tan Xi Li Street, Beijing 100050, China; Parkinson's Disease Center, Beijing Institute for Brain Disorders, #10 You'an Men Wai Xi Tou Tiao, Beijing 100069, China; China National Clinical Research Center for Neurological Diseases, #6 Tian Tan Xi Li Street, Beijing 100050, China. Electronic address: happyft@sina.com.
⁶ Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, #76 Linjiang Road, Yuzhong District, Chongqing 400010, China. Electronic address: licq9217@cqmu.edu.cn.

PMID: 26964767
DOI: 10.1016/j.neuint.2016.02.009

Abstract

Electrical stimulation of the vagus nerve, which has been used to treat epilepsy patients since 1997, also enhances long-term restoration after central nervous system (CNS) injury. Angiogenesis is a complex restorative mechanism that occurs in response to ischemic stroke, and it positively affects the recovery of neurological functions in a rat model of stroke. The aims of our study were to determine whether auricular vagus nerve stimulation (aVNS) promoted functional recovery and enhanced angiogenesis in the ischemic boundary following ischemia/reperfusion and to uncover the possible molecular mechanisms that are involved. Adult male Sprague-Dawley (SD) rats underwent transient middle cerebral artery occlusion (tMCAO) surgery and received repeated electrical stimulation of the left cavum concha starting 30 min after ischemia. For the following 21 days, we evaluated functional recovery at different time points using neurological deficit scores, the beam-walking test and the staircase test. The infarct volume was measured using TTC staining at 24 h post reperfusion, neuronal survival in the ischemic penumbra was assessed using hematoxylin and eosin (HE) staining. Microvessel density and endothelial cell proliferation in the ischemic boundary were assessed using immunofluorescence. The expression levels of brain-derived neurotrophic factor (BDNF), endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) in the ischemic penumbra were also evaluated. Our results showed that aVNS had significant neuroprotective effects and enhanced angiogenesis, which was demonstrated by improvements in the behavioral scores and brain histopathology, including increased levels of microvessel density and endothelial cell proliferation surrounding the infarct area. Furthermore, BDNF, eNOS and VEGF were expressed at higher levels in the I/R + aVNS group than in the I/R group or the I/R + sham aVNS group (p < 0.05). Our findings suggest that repeated aVNS promoted post-ischemic functional recovery and angiogenesis, possibly in conjunction with the up-regulated expression of BDNF, eNOS and VEGF in the rat brain.

Keywords: Angiogenesis; Auricular vagus nerve stimulation; BDNF; Cerebral ischemia/reperfusion; VEGF; eNOS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain-Derived Neurotrophic Factor / biosynthesis
Disease Models, Animal
Male
Neovascularization, Pathologic / metabolism*
Neovascularization, Pathologic / pathology
Neovascularization, Pathologic / therapy*
Nitric Oxide Synthase Type III / biosynthesis
Rats
Rats, Sprague-Dawley
Recovery of Function / physiology*
Reperfusion Injury / metabolism*
Reperfusion Injury / pathology
Reperfusion Injury / therapy*
Vagus Nerve Stimulation / methods*
Vascular Endothelial Growth Factor A / biosynthesis

Substances

Brain-Derived Neurotrophic Factor
Vascular Endothelial Growth Factor A
vascular endothelial growth factor A, rat
Nitric Oxide Synthase Type III
Nos3 protein, rat