Antioxidant Activity and α-Glucosidase Inhibitory Activities of the Polycondensate of Catechin with Glyoxylic Acid

PLoS One. 2016 Mar 9;11(3):e0150412. doi: 10.1371/journal.pone.0150412. eCollection 2016.

Abstract

In order to investigate polymeric flavonoids, the polycondensate of catechin with glyoxylic acid (PCG) was prepared and its chemically antioxidant, cellular antioxidant (CAA) and α-glucosidase inhibitory activities were evaluated. The DPPH and ABTS radical scavenging activities and antiproliferative effect of PCG were lower than those of catechin, while PCG had higher CAA activity than catechin. In addition, PCG had very high α-glucosidase inhibitory activities (IC50 value, 2.59 μg/mL) in comparison to catechin (IC50 value, 239.27 μg/mL). Inhibition kinetics suggested that both PCG and catechin demonstrated a mixture of noncompetitive and anticompetitive inhibition. The enhanced CAA and α-glucosidase inhibitor activities of PCG could be due to catechin polymerization enhancing the binding capacity to the cellular membrane and enzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Catechin / pharmacology*
  • Cell Proliferation / drug effects
  • Enzyme Activation / drug effects
  • Glyoxylates / pharmacology*
  • Hep G2 Cells
  • Humans
  • alpha-Glucosidases / metabolism*

Substances

  • Antioxidants
  • Glyoxylates
  • Catechin
  • alpha-Glucosidases
  • glyoxylic acid

Grants and funding

BGL received the National Natural Science Foundation of China (21166024), the Program for Innovative Research Team (in Science and Technology) in University of Henan Province (no. 13IRTSTHN006 and 16IRTSTHN007) and the Program for Science & Technology Innovation Talents in Universities of Henan Province (14HASTIT019). BGL had role in study design, data collection and analysis, decision to publish, and preparation of the manuscript.