Data on interaction between adeno-associated virus and U87 cell via cRGD chemical modification

Chuanling Zhang; Tianzhuo Yao; Yongxiang Zheng; Zhongjun Li; Lihe Zhang; Demin Zhou

doi:10.1016/j.dib.2016.02.009

Data on interaction between adeno-associated virus and U87 cell via cRGD chemical modification

Data Brief. 2016 Feb 10:7:77-80. doi: 10.1016/j.dib.2016.02.009. eCollection 2016 Jun.

Authors

Chuanling Zhang¹, Tianzhuo Yao¹, Yongxiang Zheng¹, Zhongjun Li¹, Lihe Zhang¹, Demin Zhou¹

Affiliation

¹ State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38, Xueyuan Road, Beijing 100191, China.

Abstract

RGD tripeptide is a specific, high-affinity ligand for integrin, which is highly expressed in cancer cells. We previously reported that cRGD chemically modified AAV2 (AAV2(N587+1/azido+RGD)) showed significantly enhanced infectivity compared to RGD genetically inserted AAV2 (AAV2(N587+RGD)) (10.1016/j.biomaterials.2015.11.066) [1]. Herein we provide the binding ability analysis of RGD modified AAV2 and U87 cell by flow cytometry and the theoretical working model of RGD-αvβ3 integrin interaction.

Keywords: Adeno-associated virus; Targeted gene delivery; Viral modification.