Improving the reproducibility of the NAP1/B1/027 epidemic strain R20291 in the hamster model of infection

Anaerobe. 2016 Jun:39:51-3. doi: 10.1016/j.anaerobe.2016.02.011. Epub 2016 Mar 2.

Abstract

Comparative analysis of the Clostridium difficile BI/NAP1/027 strain R20291 and ClosTron-derived ermB mutants in the hamster infection model are compromised by the clindamycin susceptibility of the parent. Mutants can appear more virulent. We have rectified this anomaly by genome engineering. The variant created (CRG20291) represents an ideal control strain for virulence assays of ClosTron mutants.

Keywords: Clindamycin sensitivity; Clostridium difficile; Genome engineering; Hamster model; ermB.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Clindamycin / pharmacology
  • Clostridioides difficile / drug effects
  • Clostridioides difficile / genetics*
  • Clostridioides difficile / pathogenicity
  • Cricetulus / genetics*
  • Cricetulus / microbiology
  • Disease Models, Animal*
  • Enterocolitis, Pseudomembranous / drug therapy
  • Enterocolitis, Pseudomembranous / microbiology*
  • Enterocolitis, Pseudomembranous / mortality
  • Enterocolitis, Pseudomembranous / pathology
  • Gene Expression
  • Genes, Synthetic*
  • Genetic Engineering
  • Genome, Bacterial*
  • Humans
  • Methyltransferases / genetics*
  • Microbial Sensitivity Tests
  • Mutation
  • Survival Analysis
  • Virulence

Substances

  • Anti-Bacterial Agents
  • Clindamycin
  • Methyltransferases
  • rRNA (adenosine-O-2'-)methyltransferase