Biological analysis and imaging applications of CdSe/CdSxSe1-x/CdS core-shell magic-sized quantum dot

Anielle Christine Almeida Silva; Ana Paula Peres Freschi; Cláudia Mendonça Rodrigues; Bruna França Matias; Larissa Prado Maia; Luiz Ricardo Goulart; Noelio Oliveira Dantas

doi:10.1016/j.nano.2016.01.001

Biological analysis and imaging applications of CdSe/CdSxSe1-x/CdS core-shell magic-sized quantum dot

Nanomedicine. 2016 Jul;12(5):1421-30. doi: 10.1016/j.nano.2016.01.001. Epub 2016 Mar 3.

Authors

Anielle Christine Almeida Silva¹, Ana Paula Peres Freschi², Cláudia Mendonça Rodrigues³, Bruna França Matias³, Larissa Prado Maia³, Luiz Ricardo Goulart⁴, Noelio Oliveira Dantas⁵

Affiliations

¹ Laboratório de Novos Materiais Isolantes e Semicondutores (LNMIS), Physics Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil. Electronic address: acalmeida@ufu.br.
² Laboratório de Novos Materiais Isolantes e Semicondutores (LNMIS), Physics Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil; Laboratório de Nanobiotecnologia, Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
³ Laboratório de Nanobiotecnologia, Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
⁴ Laboratório de Nanobiotecnologia, Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil; Department of Medical Microbiology and Immunology, University of California, Davis, Davis, CA, USA.
⁵ Laboratório de Novos Materiais Isolantes e Semicondutores (LNMIS), Physics Institute, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil. Electronic address: noelio@ufu.br.

PMID: 26945976
DOI: 10.1016/j.nano.2016.01.001

Abstract

Although colloidal magic-sized quantum dots present great promise for biological applications due to their high stability and strong luminescence, nanotoxicological analyses are scarcely reported and biomedical applications have not been demonstrated. This is the first report on biological effects of CdSe/CdSxSe1-x/CdS core-shell magic-sized quantum dot (CS-MSQD) with specific application in breast cancer cell detection. The 2-nm CS-MSQD presents a broad bandwidth emission from 450 to 750nm, low toxicity, non-immunogenicity and biocompatibility. The CS-MSQD was conjugated to a breast cancer-specific Fab antibody, and passively diffused into cells for in vitro detection of a breast cancer cell line, demonstrating to be an unprecedented tool for biomedical applications.

Keywords: Biocompatible; Bioconjugation; Biomedical imaging; Breast cancer; CdSe/CdS(x)Se(1−x)/CdS CS-MSQDs.

MeSH terms

Breast Neoplasms / diagnosis*
Cadmium Compounds*
Female
Humans
Luminescence
Luminescent Agents
Nanotechnology / methods
Quantum Dots*

Substances

Cadmium Compounds
Luminescent Agents