Photolytic Protein Aggregates: Versatile Materials for Controlled Release of Active Proteins

Akira Ishiwatari; Satoshi Yamaguchi; Satoshi Takamori; Shinya Yamahira; Kosuke Minamihata; Teruyuki Nagamune

doi:10.1002/adhm.201500957

Photolytic Protein Aggregates: Versatile Materials for Controlled Release of Active Proteins

Adv Healthc Mater. 2016 May;5(9):1002-7. doi: 10.1002/adhm.201500957. Epub 2016 Mar 4.

Authors

Akira Ishiwatari¹, Satoshi Yamaguchi², Satoshi Takamori¹, Shinya Yamahira¹, Kosuke Minamihata¹, Teruyuki Nagamune^{1

3}

Affiliations

¹ Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
² Research Center for Advanced Science and Technology (RCAST), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan.
³ Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.

PMID: 26945901
DOI: 10.1002/adhm.201500957

Abstract

Photolytic protein aggregates are developed as a facile and versatile platform for light-induced release of active proteins. The proteins modified with biotin through a photo-cleavable linker rapidly form aggregates with streptavidin and biotinylated functional molecules simply by mixing. Light irradiation releases active proteins from the aggregates in high yields, and light-induced uptake of drug-modified transferrin into living cells is successfully demonstrated.

Keywords: biomaterials;·drug delivery; mammalian cells; photolysis; protein engineering.

MeSH terms

Cell Line
Delayed-Action Preparations / pharmacokinetics
Delayed-Action Preparations / pharmacology
Humans
Photolysis*
Protein Aggregation, Pathological* / drug therapy
Protein Aggregation, Pathological* / metabolism
Protein Aggregation, Pathological* / pathology
Transferrin* / pharmacokinetics
Transferrin* / pharmacology

Substances

Delayed-Action Preparations
Transferrin