The implications of endemic IMP-4 carbapenemase for clinical laboratory susceptibility testing

Namraj Goire; Gerald B Harnett; Lyn C O'Reilly; Paul R Ingram; Michael J Leung; David J Speers; Paul E Healy; Timothy J J Inglis

doi:10.1016/j.mimet.2016.03.001

The implications of endemic IMP-4 carbapenemase for clinical laboratory susceptibility testing

J Microbiol Methods. 2016 May:124:10-2. doi: 10.1016/j.mimet.2016.03.001. Epub 2016 Mar 2.

Authors

Namraj Goire¹, Gerald B Harnett¹, Lyn C O'Reilly², Paul R Ingram³, Michael J Leung⁴, David J Speers⁵, Paul E Healy¹, Timothy J J Inglis⁴

Affiliations

¹ Department of Microbiology, PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Hospital Avenue, Nedlands, Western Australia 6009, Australia.
² Department of Microbiology, PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Hospital Avenue, Nedlands, Western Australia 6009, Australia. Electronic address: Lyn.O'Reilly@health.wa.gov.au.
³ Department of Microbiology, PathWest Laboratory Medicine WA, Fiona Stanley Hospital, Perth, Western Australia 6150, Australia; School of Pathology and Laboratory Medicine, University of Western Australia, Crawley, Western Australia 6009, Australia.
⁴ Department of Microbiology, PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Hospital Avenue, Nedlands, Western Australia 6009, Australia; School of Pathology and Laboratory Medicine, University of Western Australia, Crawley, Western Australia 6009, Australia.
⁵ Department of Microbiology, PathWest Laboratory Medicine WA, Queen Elizabeth II Medical Centre, Hospital Avenue, Nedlands, Western Australia 6009, Australia; School of Medicine and Pharmacology, University of Western Australia, Crawley, Western Australia 6009, Australia.

PMID: 26945518
DOI: 10.1016/j.mimet.2016.03.001

Abstract

A local predominance of carbapenemase producing Enterobacteriaceae with low minimum inhibitory concentrations (MIC) to meropenem prompted a review of methods available for carbapenemase detection. We report on results using two selective media, temocillin discs, CarbaNP test, GeneXpert Carba-R assay and an in-house PCR assay.

Keywords: CarbaNP test; Carbapenemase producing Enterobacteriaceae; GeneXpert Carba-R assay.

Publication types

Evaluation Study

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / analysis*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Enterobacteriaceae / drug effects
Enterobacteriaceae / enzymology*
Enterobacteriaceae / genetics
Enterobacteriaceae Infections / microbiology*
Enzyme Assays / methods*
Humans
Meropenem
Microbial Sensitivity Tests
Polymerase Chain Reaction / methods*
Thienamycins / pharmacology
beta-Lactamases / analysis*
beta-Lactamases / genetics
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
Bacterial Proteins
Thienamycins
beta-lactamase IMP-4
beta-Lactamases
Meropenem