Silica sol assisted chromatographic NMR spectroscopy for resolution of trans- and cis-isomers

Ying Yang; Rui Wu; Shaohua Huang; Zhengwu Bai

doi:10.1016/j.jmr.2016.02.013

Silica sol assisted chromatographic NMR spectroscopy for resolution of trans- and cis-isomers

J Magn Reson. 2016 Apr:265:210-4. doi: 10.1016/j.jmr.2016.02.013. Epub 2016 Feb 27.

Authors

Ying Yang¹, Rui Wu², Shaohua Huang³, Zhengwu Bai²

Affiliations

¹ Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, 266101 Qingdao, China.
² School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, 693 Xiongchu Avenue, 430073 Wuhan, China.
³ Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, 266101 Qingdao, China. Electronic address: huangsh@qibebt.ac.cn.

PMID: 26942864
DOI: 10.1016/j.jmr.2016.02.013

Abstract

Chromatographic NMR spectroscopy can separate the mixtures of species with significantly different molecular size, but generally fails for isomeric species. Herein, we reported the resolution of trans- and cis-isomers and their structural analogue, which are different in molecular shapes, but similar in mass, were greatly enhanced in the presence of silica sol. The mixtures of maleic acid, fumaric acid and succinic acid, and the mixtures of trans- and cis-1,2-cyclohexanedicarboxylic acids, were distinguished by virtue of their different degrees of interaction with silica sol. Moreover, we found mixed solvents could improve the spectral resolution of DOSY spectra of mixtures.

Keywords: Chromatographic NMR spectroscopy; DOSY; Isomer; Resolution; Silica sol.

Publication types

Research Support, Non-U.S. Gov't