Recombinant Measles AIK-C Vaccine Strain Expressing the prM-E Antigen of Japanese Encephalitis Virus

PLoS One. 2016 Mar 1;11(3):e0150213. doi: 10.1371/journal.pone.0150213. eCollection 2016.

Abstract

An inactivated Japanese encephalitis virus (JEV) vaccine, which induces neutralizing antibodies, has been used for many years in Japan. In the present study, the JEV prM-E protein gene was cloned, inserted at the P/M junction of measles AIK-C cDNA, and an infectious virus was recovered. The JEV E protein was expressed in B95a cells infected with the recombinant virus. Cotton rats were inoculated with recombinant virus. Measles PA antibodies were detected three weeks after immunization. Neutralizing antibodies against JEV developed one week after inoculation, and EIA antibodies were detected three weeks after immunization. The measles AIK-C-based recombinant virus simultaneously induced measles and JEV immune responses, and may be a candidate for infant vaccines. Therefore, the present strategy of recombinant viruses based on a measles vaccine vector would be applicable to the platform for vaccine development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Neutralizing
  • Antibodies, Viral / immunology
  • Chlorocebus aethiops
  • Encephalitis Virus, Japanese / immunology*
  • Encephalitis, Japanese / prevention & control
  • Female
  • HEK293 Cells
  • Humans
  • Japanese Encephalitis Vaccines / immunology*
  • Measles / prevention & control
  • Measles Vaccine / immunology*
  • Measles virus / immunology*
  • Rats
  • Vaccines, Synthetic / immunology*
  • Vero Cells

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Japanese Encephalitis Vaccines
  • Measles Vaccine
  • Vaccines, Synthetic

Grants and funding

TN had research funding from Daiichi Sankyo, and some parts of the study were supported by the fund. Daiichi Sankyo provided support in the form of salaries for authors AH, HT, and TK and provided JEV vaccine strain, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the author contributions section.