Motility of carp spermatozoa is associated with profound changes in the sperm proteome

Abstract

In freshwater cyprinids, spermatozoa are quiescent in seminal plasma and sperm motility is initiated by a decrease in osmolality (hypo-osmotic shock) after discharge into the aqueous environment. However, it is unknown at present if and to what extent changes in proteins are involved in carp sperm motility. Therefore, the aim of our study was to identify proteins related to carp sperm motility through a comparison of immobilized and activated carp spermatozoa using a 2D-DIGE approach. Our results, for the first time indicated that carp sperm motility is associated with changes in protein content. Seventy-two differentially expressed proteins were identified. These proteins are mainly involved in ubiquitin-proteasome pathways, glycolysis, the TCA cycle, remodeling and are putatively related to sperm energy metabolism and motility. Moreover proteins associated with oxidative stress responses, signal transduction by Ca(2+)-dependent MAPK cascades, and PKC and protein folding have been identified. The proteins involved in carp sperm motility were localized to the cytoplasm, mitochondria, cytoskeleton, nucleus and sperm membrane. The identification of a high number of proteins involved in carp sperm motility would contribute to current knowledge about the molecular mechanisms of sperm motility in freshwater fish.

Biological significance: To the best of our knowledge, few changes in proteins involved in the initiation of fish sperm motility have been identified. This is a limited number of proteins compared with the 80 recently identified proteins involved in human sperm motility. However, no proteomic studies of sperm motility have yet been performed on freshwater fish. Our present study allowed for the first time a comprehensive characterization of the proteins associated with carp sperm motility and a better understanding of the molecular mechanisms underlying sperm motility activation and maintenance. The application of 2D-DIGE facilitated the identification proteins crucial for sperm structural organization and motility. The identification of a high number of proteins involved in carp sperm motility would contribute appreciably to the presently limited information available on the mechanisms of sperm motility in freshwater fish. Moreover the identified list of proteins will create a platform for future studies designed to assess the functional significance of specific proteins in sperm motility.

Keywords: Carp; Motility; Proteome; Spermatozoa.