C-type natriuretic peptide improved vitrified-warmed mouse cumulus oocyte complexes developmental competence

Cryobiology. 2016 Apr;72(2):161-4. doi: 10.1016/j.cryobiol.2016.02.002. Epub 2016 Feb 24.

Abstract

Cryopreservation of cumulus oocyte complexes (COCs) is important for reproductive medicine. However, the vitrified-warmed COCs have lower maturation rate and subsequent developmental competence compared with fresh COCs. The present study was aimed to evaluate the effects of supplementation of the maturation medium with C-type natriuretic peptide (CNP) on the developmental competence of vitrified-warmed mouse COCs. Addition of CNP to the maturation medium improved the maturation rate and enhanced the developmental competence of vitrified-warmed mouse COCs. The reason may be that vitrified COCs led to a decline in cyclic guanosine monophosphate (cGMP) levels. Furthermore, addition of CNP to the maturation medium elevated cGMP levels of the vitrified-warmed COCs. In conclusion, cryopreservation-associated lower maturation rate and developmental competence of COCs may be ameliorated by CNP during maturation culture after warming.

Keywords: C-type natriuretic peptide; Cumulus oocyte complexes; Cyclic guanosine monophosphate; Vitrification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cryopreservation / methods*
  • Cumulus Cells / cytology*
  • Cumulus Cells / drug effects
  • Cyclic GMP / metabolism
  • Mice
  • Natriuretic Peptide, C-Type / pharmacology*
  • Oocytes / cytology*
  • Oocytes / drug effects
  • Oogenesis / drug effects*
  • Vitrification*

Substances

  • Natriuretic Peptide, C-Type
  • Cyclic GMP