Structural confirmation of novel oligosaccharides isolated from sugar beet molasses

Tatsuya Abe; Hiroto Kikuchi; Tsutomu Aritsuka; Yusuke Takata; Eri Fukushi; Yukiharu Fukushi; Jun Kawabata; Keiji Ueno; Shuichi Onodera; Norio Shiomi

doi:10.1016/j.foodchem.2016.01.126

Structural confirmation of novel oligosaccharides isolated from sugar beet molasses

Food Chem. 2016 Jul 1:202:284-90. doi: 10.1016/j.foodchem.2016.01.126. Epub 2016 Jan 28.

Authors

Tatsuya Abe¹, Hiroto Kikuchi², Tsutomu Aritsuka³, Yusuke Takata⁴, Eri Fukushi⁵, Yukiharu Fukushi⁶, Jun Kawabata⁷, Keiji Ueno⁸, Shuichi Onodera⁹, Norio Shiomi¹⁰

Affiliations

¹ Research Center, Nippon Beet Sugar Mfg. Co., Ltd., Obihiro 080-0831, Japan. Electronic address: abet@nitten.co.jp.
² Research Center, Nippon Beet Sugar Mfg. Co., Ltd., Obihiro 080-0831, Japan. Electronic address: kikuti@nitten.co.jp.
³ Research Center, Nippon Beet Sugar Mfg. Co., Ltd., Obihiro 080-0831, Japan. Electronic address: arit@nitten.co.jp.
⁴ Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address: kitto56@abs.agr.hokudai.ac.jp.
⁵ Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address: feria@cen.agr.hokudai.ac.jp.
⁶ Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address: y-fuku@abs.agr.hokudai.ac.jp.
⁷ Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address: junk@chem.agr.hokudai.ac.jp.
⁸ Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University, Ebetsu 069-8501, Japan. Electronic address: ueno-k@rakuno.ac.jp.
⁹ Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University, Ebetsu 069-8501, Japan. Electronic address: sdera@rakuno.ac.jp.
¹⁰ Department of Food and Nutrition Sciences, Graduate School of Dairy Science Research, Rakuno Gakuen University, Ebetsu 069-8501, Japan. Electronic address: nsalt@frontier.hokudai.ac.jp.

PMID: 26920296
DOI: 10.1016/j.foodchem.2016.01.126

Abstract

Eleven oligosaccharides were isolated from sugar beet molasses using carbon-Celite column chromatography and HPLC. The constituent sugars and linkage positions were determined using methylation analysis, MALDI-TOF-MS, and NMR measurements. The configurations of isolated oligosaccharides were confirmed based on detailed NMR analysis. Based on our results, three of the 11 oligosaccharides were novel.

Keywords: Beet molasses; NMR; Oligosaccharide.

MeSH terms

Beta vulgaris / chemistry*
Chromatography, High Pressure Liquid
Magnetic Resonance Spectroscopy
Molasses / analysis*
Oligosaccharides / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

Oligosaccharides