Multigrowth Factor Delivery via Immobilization of Gene Therapy Vectors

Jie Hao; Kenneth C K Cheng; Laura G Kruger; Lena Larsson; James V Sugai; Joerg Lahann; William V Giannobile

doi:10.1002/adma.201600027

Multigrowth Factor Delivery via Immobilization of Gene Therapy Vectors

Adv Mater. 2016 Apr;28(16):3145-51. doi: 10.1002/adma.201600027. Epub 2016 Feb 25.

Authors

Jie Hao¹, Kenneth C K Cheng², Laura G Kruger¹, Lena Larsson^{1

3}, James V Sugai¹, Joerg Lahann⁴, William V Giannobile⁵

Affiliations

¹ Periodontics and Oral Medicine, School of Dentistry, University of Michigan, 1011 North University Avenue, Ann Arbor, MI, 48109-1078, USA.
² Biointerfaces Institute, Department of Materials Science and Engineering, B26-115S NCRC, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI, 48109-2800, USA.
³ Department of Periodontology, Institute of Odontology, Medicinaregatan 12F, 6th Floor, University of Gothenburg, SE-405 30 Gothenburg, Sweden.
⁴ Biointerfaces Institute, Department of Chemical Engineering, Materials Science and Engineering, Biomedical Engineering, Macromolecular Science and Engineering, B10-A175 NCRC, University of Michigan, 2800 Plymouth Road, Ann Arbor, MI, 48109-2800, USA.
⁵ Department of Periodontics and Oral Medicine and Department of Biomedical Engineering, University of Michigan, 1011 North University Avenue, Ann Arbor, MI, 48109-1078, USA.

Abstract

Molecules can be immobilized onto biomaterials by a chemical vapor deposition (CVD) coating strategy. Pentafluorophenolester groups react with amine side chains on antibodies, which can selectively immobilize adenoviral vectors for gene delivery of growth factors. These vectors can produce functional proteins within defined regions of biomaterials to produce customizable structures for targeted tissue regeneration.

Keywords: bone morphogenetic proteins; drug delivery; gene transfer; platelet-derived growth factor; regenerative medicine; tissue regeneration.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Adenoviridae / genetics*
Adenoviridae / immunology
Antibodies, Immobilized / chemistry*
Biocompatible Materials / chemistry*
Cells, Cultured
Gene Transfer Techniques*
Genetic Therapy / methods*
Genetic Vectors* / genetics
Genetic Vectors* / immunology
Humans
Intercellular Signaling Peptides and Proteins / biosynthesis
Intercellular Signaling Peptides and Proteins / genetics*
Periodontal Ligament / cytology
Regenerative Medicine / methods
Wound Healing

Substances

Antibodies, Immobilized
Biocompatible Materials
Intercellular Signaling Peptides and Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding