Exploiting Copper Redox for (19)F Magnetic Resonance-Based Detection of Cellular Hypoxia

Da Xie; Tyler L King; Arnab Banerjee; Vikraant Kohli; Emily L Que

doi:10.1021/jacs.5b13215

Exploiting Copper Redox for (19)F Magnetic Resonance-Based Detection of Cellular Hypoxia

J Am Chem Soc. 2016 Mar 9;138(9):2937-40. doi: 10.1021/jacs.5b13215. Epub 2016 Feb 26.

Authors

Da Xie¹, Tyler L King¹, Arnab Banerjee¹, Vikraant Kohli¹, Emily L Que¹

Affiliation

¹ Department of Chemistry, The University of Texas at Austin , 105 E. 24th St Stop A5300, Austin, Texas 78712, United States.

PMID: 26906216
DOI: 10.1021/jacs.5b13215

Abstract

We report a pair of fluorinated, redox-active copper complexes for potential use as (19)F MRI contrast agents for detecting cellular hypoxia. Trifluorinated Cu(II) ATSM-F3 displays the appropriate redox potential for selective accumulation in hypoxic cells and a completely quenched (19)F NMR signal that is "turned on" following reduction to Cu(I). Incubation of cancer cells with CuATSM-F3 resulted in a selective detection of (19)F signal in cells grown under hypoxic conditions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Hypoxia / physiology*
Coordination Complexes / chemistry*
Copper / chemistry*
Crystallography, X-Ray
Fluorine-19 Magnetic Resonance Imaging / methods*
Humans
Ligands
MCF-7 Cells
Organometallic Compounds / chemistry*
Oxidation-Reduction
Thiosemicarbazones / chemistry*

Substances

Coordination Complexes
Ligands
Organometallic Compounds
Thiosemicarbazones
copper (II) diacetyl-di(N(4)-methylthiosemicarbazone)
Copper