Quantitative PCR studies of Aggregatibacter actinomycetemcomitans and Treponema denticola/Tanerella forsythensis Complex as Etiological Factors of Chronic Periodontitis

O O Yanushevich; R A Ayvazova; A V Shibaeva; D V Rebrikov; E V Trubnikova; Yu K Kudykina; M V Zylnikova; R S Zaripova; A B Shevelev

doi:10.1007/s10517-016-3205-9

Quantitative PCR studies of Aggregatibacter actinomycetemcomitans and Treponema denticola/Tanerella forsythensis Complex as Etiological Factors of Chronic Periodontitis

Bull Exp Biol Med. 2016 Feb;160(4):495-7. doi: 10.1007/s10517-016-3205-9. Epub 2016 Feb 22.

Authors

O O Yanushevich¹, R A Ayvazova², A V Shibaeva^{3

4}, D V Rebrikov⁵, E V Trubnikova⁶, Yu K Kudykina⁴, M V Zylnikova^{4

7}, R S Zaripova⁷, A B Shevelev⁴

Affiliations

¹ A. I. Evdokimov Moscow State University of Medicine and Dentistry, the Ministry of Health of the Russian Federation, Moscow, Russia.
² A. I. Evdokimov Moscow State University of Medicine and Dentistry, the Ministry of Health of the Russian Federation, Moscow, Russia. a_reg@mail.ru.
³ N. M. Emanuel Institute of Biochemical Physics, the Russian Academy of Sciences, Moscow, Russia.
⁴ M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, the Russian Academy of Medical Sciences, Moscow, Russia.
⁵ N. I. Vavilov Institute of Common Genetics, the Russian Academy of Sciences, Moscow, Russia.
⁶ Kursk State University, Kursk, Russia.
⁷ Naberezhnye Chelny Institute of Socio-Pedagogical Technologies and Resources, Naberezhnye Chelny, Russia.

PMID: 26899837
DOI: 10.1007/s10517-016-3205-9

Abstract

Real-time quantitative PCR (Dentofl or kit) was used to detect DNA of periodontal pathogens in specimens from 92 patients with chronic periodontitis and from a control sample of 12 normal subjects. A bimodal distribution of patients by periodontium colonization with A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythensis, and T. denticola was demonstrated. A new approach to interpretation of the results of quantitative evaluation of periodontal pathogens, including the notion of pathological colonization level, led to classification of all cases with chronic generalized periodontitis into 3 groups: associated with A. actinomycetemcomitans, with T. forsythensis/T. denticola complex, and cases of uncertain genesis.

Keywords: Aggregatibacter actinomycetemcomitans; Dentoflor; Tanerella forsythensis; Treponema denticola; periodontitis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Aged
Aged, 80 and over
Aggregatibacter actinomycetemcomitans / isolation & purification*
Chronic Periodontitis / microbiology*
Humans
Middle Aged
Periodontium / microbiology*
Periodontium / pathology
Porphyromonas gingivalis / isolation & purification*
Real-Time Polymerase Chain Reaction
Tannerella forsythia / isolation & purification*
Treponema denticola / isolation & purification*
Young Adult