Comparison of fluorescent tags for analysis of mannose-6-phosphate glycans

Anal Biochem. 2016 May 15:501:1-3. doi: 10.1016/j.ab.2016.02.004. Epub 2016 Feb 11.

Abstract

Mannose-6-phosphate (M-6-P) glycan analysis is important for quality control of therapeutic enzymes for lysosomal storage diseases. Here, we found that the analysis of glycans containing two M-6-Ps was highly affected by the hydrophilicity of the elution solvent used in high-performance liquid chromatography (HPLC). In addition, the performances of three fluorescent tags--2-aminobenzoic acid (2-AA), 2-aminobenzamide (2-AB), and 3-(acetyl-amino)-6-aminoacridine (AA-Ac)--were compared with each other for M-6-P glycan analysis using HPLC and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The best performance for analyzing M-6-P glycans was shown by 2-AA labeling in both analyses.

Keywords: Fluorescent labeling tag; Glycan analysis; High-performance liquid chromatography (HPLC); Mannose-6-phosphate (M-6-P); Matrix-assisted laser desorption/ionization time-of-flight (MALDI–TOF) mass spectrometry.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminacrine / analogs & derivatives
  • Aminobenzoates / chemistry
  • Chromatography, High Pressure Liquid / methods
  • Fluorescent Dyes / chemistry*
  • Hydrophobic and Hydrophilic Interactions
  • Mannosephosphates / analysis*
  • Polysaccharides / chemistry*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • ortho-Aminobenzoates / chemistry

Substances

  • Aminobenzoates
  • Fluorescent Dyes
  • Mannosephosphates
  • Polysaccharides
  • ortho-Aminobenzoates
  • mannose-6-phosphate
  • Aminacrine
  • anthranilamide