Exogenous control over intracellular acidification: Enhancement via proton caged compounds coupled to gold nanoparticles and an alternative pathway with DMSO

Marilena Carbone; Gianfranco Sabbatella; Simonetta Antonaroli; Hynd Remita; Viviana Orlando; Stefano Biagioni; Alessandro Nucara

doi:10.1016/j.dib.2015.12.032

Exogenous control over intracellular acidification: Enhancement via proton caged compounds coupled to gold nanoparticles and an alternative pathway with DMSO

Data Brief. 2016 Jan 21:6:745-9. doi: 10.1016/j.dib.2015.12.032. eCollection 2016 Mar.

Authors

Marilena Carbone¹, Gianfranco Sabbatella², Simonetta Antonaroli³, Hynd Remita⁴, Viviana Orlando⁵, Stefano Biagioni⁵, Alessandro Nucara⁶

Affiliations

¹ Dept. of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1-00133 Rome, Italy; Consorzio Interuniversitario Biostrutture e Biosistemi, Viale Medaglie d'Oro 305, 00136 Rome, Italy.
² Dept. of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1-00133 Rome, Italy; Dept. of Chemistry, University of Rome La Sapienza, P.le A. Moro, 00185 Rome, Italy.
³ Dept. of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1-00133 Rome, Italy.
⁴ Laboratoire de Chimie Physique, UMR 8000-CNRS, Bât. 349, Université Paris-Sud, 91405 Orsay, France.
⁵ Dept. of Biology and Biotechnology "Charles Darwin", University of Rome La Sapienza, P.le A. Moro, 00185 Rome, Italy.
⁶ Dept. of Physics, University of Rome La Sapienza, P.le A. Moro, 00185 Rome, Italy; Center for Life Nano Science, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161 Rome, Italy.

Abstract

Proton caged compounds exhibit a characteristic behavior when directly dosed into cells or being coupled to gold nanoparticles prior to the dosing. When irradiated in the near ultraviolet region, they release protons that interact with intracellular HCO3 (-) to yield H2CO3. The dissociation of carbonic acid, then, releases CO2 that can be distinctively singled out in infrared spectra. In the process of searching a pathway to augment the intracellular uptake of proton caged compounds, we probed the association of 1-(2-nitrophenyl)-ethylhexadecyl sulfonate (HDNS) with DMSO, an agent to enhance the membrane permeability. We found out a different UV-induced protonation mechanism that opens up to new conduits of employing of proton caged compounds. Here, we report the infrared data we collected in this set of experiments.

Keywords: DMSO; Intracellular proton release; Proton caged compounds.