Evaluation of GenoFlow DR-MTB Array Test for Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis

B Molina-Moya; G Kazdaglis; A Lacoma; C Prat; A Gómez; R Villar-Hernández; E García-García; L Haba; J Maldonado; S Samper; J Ruiz-Manzano; J Domínguez

doi:10.1128/JCM.03341-15

Evaluation of GenoFlow DR-MTB Array Test for Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis

J Clin Microbiol. 2016 Apr;54(4):1160-3. doi: 10.1128/JCM.03341-15. Epub 2016 Feb 10.

Authors

B Molina-Moya¹, G Kazdaglis², A Lacoma¹, C Prat¹, A Gómez¹, R Villar-Hernández¹, E García-García¹, L Haba³, J Maldonado⁴, S Samper⁵, J Ruiz-Manzano⁶, J Domínguez⁷

Affiliations

¹ Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain.
² Department of Medical Microbiology, Aristotle University of Thessaloniki, Thessaloniki, Greece.
³ Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain.
⁴ Serveis Clínics, Barcelona, Spain.
⁵ CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain Hospital Universitario Miguel Servet, Zaragoza, Spain.
⁶ Servei de Pneumologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain.
⁷ Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain jadomb@gmail.com.

Abstract

The aim of this study was to evaluate the GenoFlow DR-MTB array test (DiagCor Bioscience, Hong Kong) on 70 cultured isolates and 50 sputum specimens. The GenoFlow array test showed good sensitivity and specificity compared to the phenotypic Bactec 460TB. This array accurately detected mutations inrpoB,katG, andinhAassociated with resistance to rifampin and isoniazid.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Antitubercular Agents / pharmacology*
Bacterial Proteins / genetics
Catalase / genetics
Drug Resistance, Bacterial*
Genotype
Genotyping Techniques / methods*
Humans
Isoniazid / pharmacology*
Mutation
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics*
Oxidoreductases / genetics
RNA Polymerase II / genetics
Rifampin / pharmacology*
Sensitivity and Specificity

Substances

Antitubercular Agents
Bacterial Proteins
Oxidoreductases
Catalase
katG protein, Mycobacterium tuberculosis
InhA protein, Mycobacterium
RNA Polymerase II
Isoniazid
Rifampin

Grants and funding

J. Domínguez is funded by the Miguel Servet program of the Instituto de Salud Carlos III (Spain). The research was partially supported by a grant from the Instituto de Salud Carlos III (PI 13/01546). The funders had no role in the study design, data collection and interpretation, or the decision to submit the work for publication.