Inactivating Zaire Ebolavirus in Whole-Blood Thin Smears Used for Malaria Diagnosis

Todd A. Cutts; Bradley W. M. Cook; Guillaume Poliquin; James E. Strong; Steven S Theriault

doi:10.1128/JCM.02960-15

Inactivating Zaire Ebolavirus in Whole-Blood Thin Smears Used for Malaria Diagnosis

J Clin Microbiol. 2016 Apr;54(4):1157-9. doi: 10.1128/JCM.02960-15. Epub 2016 Feb 10.

Authors

Todd A. Cutts¹, Bradley W. M. Cook², Guillaume Poliquin³, James E. Strong⁴, Steven S Theriault⁵

Affiliations

¹ Applied Biosafety Research Program, Canadian Science Centre for Human and Animal Health Winnipeg, Manitoba, Canada J. C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
² J. C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Manitoba, Canada Applied Biosafety Research Program, Canadian Science Centre for Human and Animal Health Winnipeg, Manitoba, Canada.
³ Office of the Scientific Director General, Canadian Science Centre for Human and Animal Health, Public Health Agency of Canada, Winnipeg, Manitoba, Canada Department of Pediatrics and Child Health, The University of Manitoba, Winnipeg, Manitoba, Canada.
⁴ Department of Pediatrics and Child Health, The University of Manitoba, Winnipeg, Manitoba, Canada Diagnostics Unit, Special Pathogens Program, Canadian Science Centre for Human and Animal Health, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
⁵ Applied Biosafety Research Program, Canadian Science Centre for Human and Animal Health Winnipeg, Manitoba, Canada J. C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Manitoba, Canada Department of Microbiology, The University of Manitoba, Winnipeg, Manitoba, Canada steven.theriault@phac-aspc.gc.ca.

Abstract

Malaria is an important mimic or coinfection in potential Ebolavirus disease patients. Here, we evaluated the efficacy of the 100% methanol-inactivating Zaire Ebolavirus Makona variant for malaria thin-smear preparation. We determined that 100% methanol completely inactivated the virus after 15 min.

MeSH terms

Blood / virology*
Disinfectants / pharmacology*
Disinfection / methods*
Ebolavirus / drug effects*
Ebolavirus / physiology
Humans
Malaria / diagnosis*
Methanol / pharmacology*
Microbial Viability / drug effects*
Time Factors

Substances

Disinfectants
Methanol